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European Calcified Tissue Society Congress 2013

Lisbon, Portugal
18 May 2013 - 22 May 2013

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European Calcified Tissue Society Congress 2013, 18 - 22 May 2013; Lisbon, Portugal

Poster Presentations

Calciotropic and phosphotropic hormones and mineral metabolism

ba0001pp99 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Interrelationship between osteocalcin (bgp) and energetic metabolism in two strains of rats

Marotte Clarisa , Bryk Gabriel , Coronel Magali Zeni , Abrego Paulina , Lucero Diego Martin , Schreier Laura , Portela Maria Luz , Zeni Susana Noemi

It has suggested that BGP is inversely related to body fat. BGP appears to regulate energetic metabolism through the insulin receptor signaling pathway in the osteoblast, which affects bone resorption and BGP activity. Fat tissue is an endocrine organ that secretes different hormones and growth factors; several of them affect bone remodeling and insulin secretion. The present report comparatively studied total BGP levels, glucose homeostasis and body fat mass in spontaneous ob...

ba0001pp100 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

A mixture of GOS/FOS ® added to a low calcium (ca) diet improved ca, phosphorus (p) and magnesium (mg) absorption: experimental model in normal growing rats

Bryk Gabriel , Chaves Macarena Gonzales , Marotte Clarisa , Medina Daniela , Coronel Magali Zeni , de Portela Maria Luz , Zeni Susana Noemi

A mixture of Galacto-oligosaccharides (GOS) and Fructooligosaccharides (FOS) are added to commercial infant formula to promote an intestinal microbiota similar to that prevalent in breast-fed infants to improve Ca bioavailability and general health, but their mechanisms are under debate.Our objective was to evaluate the beneficial effects of the mixture of GOS/FOS added to infant formulae, on the absorption of Ca, Mg and P of a low Ca diet. Changes in in...

ba0001pp101 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Vasculature and bone: stages of atherosclerosis come along with changes in gene expression levels of calcification regulators

Schweighofer Natascha , Aigelsreiter Ariane , Graf-Rechberger Martina , Hacker Nicole , Kniepeiss Daniela , Stiegler Philipp , Trummer Olivia , Pieber Thomas , Ulbing Matthias , Wagner Doris , Muller Helmut , Obermayer-Pietsch Barbara

Calcification in the vasculature is one of the leading causes of cardiovascular diseases and mortality outcomes. Therefore, the aim of our study was to investigate changes in the gene expression of calcification regulators (CR) in arterial vessels during different stages of atherosclerosis and to document potential corresponding changes in the bone. OPG, RANKL, OPN, MGP, BSP-II and RUNX2 were candidate genes for our study in bone, aorta and arteria ilica externa tissue samples...

ba0001pp102 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

A genetic polymorphism of osteocalcin is associated with BMI but not with parameters of glucose and lipid metabolism in women with polycystic ovary syndrome

Schwetz Verena , Trummer Olivia , Giuliani Albrecht , Pieber Thomas R , Obermayer-Pietsch Barbara , Lerchbaum Elisabeth

Introduction: Osteocalcin (OC) is a marker of bone formation but also seems to play a hormonal role in the regulation of glucose and energy metabolism. Recently, an association of BMI with a haplotype composed of three single nucleotide polymorphisms (SNPs) in the gene for OC, located on chromosome 1q22, was observed in ethnically homogeneous European pedigrees.Aim: The aim of the study was to test the association of these three polymorphisms in the gene...

ba0001pp104 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Neonatal neuroendocrine alterations impair tooth eruption, enamel mineralization, and leptin and corticosterone secretion in adulthood

de Mello Wagner Garcez , de Morais Samuel Rodrigues Lourenco , Delbem Alberto Carlos Botazzo , Dornelles Rita Cassia Menegati , Antunes-Rodrigues Jose , de Castro Joao Cesar Bedran

There is a growing body of evidence indicating the important role of the neonatal steroid milieu in programming sexually dimorphic pattern in various physiological systems. We tested the hypothesis that abnormal exposure to steroid hormones within a critical developmental period elicits permanent changes on tooth eruption, enamel mineralization, and leptin and corticosterone concentrations in adulthood. Newborn Wistar rats were divided into four groups, two male groups and two...

ba0001pp105 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

α2C AR KO mice present opposite bone phenotype in femur and vertebrae

Teixeira Marilia Bianca , Martins Gisele , Costa Cristiane , Brum Patricia , Gouveia Cecilia

α2C Evidences demonstrate that sympathetic nervous system (SNS) activation causes osteopenia via β2-adrenoceptor (β2-AR) signaling. In a recent study, we showed that female mice with chronic sympathetic hyperactivity due to double knockout of adrenoceptors that negatively regulate norepinephrine release, α2A-AR and α2C-AR (α2A/α2C-ARKO), present an unexpected phenoty...

ba0001pp106 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Bone microtomography and immunohistochemistry of acyclic rats post antiresorptive therapy and resistance training

Stringhetta-Garcia Camila , Morais Samuel , Louzada Mario , Ervolino Edilson , Dornelles Rita

Osteoporosis is a growing public health problem. As allies have for preventing physical activity, especially resistance training (RT) and hormone replacement. The aim of this study was to analyze the effect of Raloxifene (RLX) and RT in bone metabolism of rats on their aging period. Wistar rats (14–18 months), sham or ovariectomized (OVX) were treated with RLX (1 mg/kg per day) or saline by gavage and subjected to RT or not. The animals were subjected to RT-oriented pract...

ba0001pp108 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

A sensitive assay for measuring circulating BMP6

Pauk Martina , Grgurevic Lovorka , Brkljacic Jelena , Kufner Vera , Bordukalo-Niksic Tatjana , Jankolija Morana , Oppermann Hermann , Vukicevic Slobodan

Although bone morphogenetic protein 6 (BMP6) is known for its ability to induce growth of bone and cartilage, recent studies identified BMP6 as a key endogenous regulator of hepcidin expression and iron metabolism. Here, we examined BMP6 presence in the serum and investigated whether circulating levels of BMP6 may reflect body iron status. First, we showed by liquid chromatography–mass spectrometry (LC–MS) and western blotting that BMP6 is present in the circulation ...

ba0001pp109 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Biochemical bone markers of bone turnover in diabetics: a meta-analysis

Starup-Linde Jakob , Eriksen Stine Aistrup , Vestergaard Peter

Introduction: Diabetes mellitus may affect bone via bone structure, bone density, and biochemical markers of bone turnover. The aim of this meta-analysis was to examine biochemical markers of bone turnover in diabetics compared to non-diabetics.Methods: A systematic literature search was conducted using Pubmed, Embase, Cinahl, and Svemed+ with the search terms: ‘Diabetes mellitus’, ‘Diabetes mellitus type 1’, ‘Insulin dependent d...

ba0001pp110 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Short-term resveratrol supplementation stimulates serum levels of bone-specific alkaline phosphatase in obese non-diabetic men

Poulsen Morten Moller , Ornstrup Marie Juul , Harslof Torben , Jessen Niels , Langdahl Bente Lomholt , Richelsen Bjorn , Lunde Jorgensen Jens Otto , Pedersen Steen Bonlokke

Despite the substantial preclinical evidence for a positive effect of the polyphenolic compound resveratrol, human data are very scarce, and currently no clinical data addressing the potential impact on bone metabolism have been published. In the present study we addressed this issue by testing a panel of bone specific biomarkers in order to identify potential bone metabolic effects of resveratrol in human subjects. In a randomized, placebo-controlled, double-blinded and paral...

ba0001pp111 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

The effect of gastric bypass treatment for obesity on hormones related to bone re-modeling and intestinal growth

Hartmann Bolette , Vestergaard Henrik , Bonfils Peter , Hansen Marie , Albrechtsen Nicolai , Eskildsen Peter , Floyd Andrea , Svenningsen Annette Lykke , Holst Jens Juul

Aim: The aim of the study was to investigate the relationship between gut hormones and bone re-modeling and intestinal growth by measuring meal stimulated changes in hormones and bone markers in blood samples collected from patients before and after gastric bypass (GBP).Methods: Eighteen patients (12 females and 6 males) were included in the study, approved by the Municipal Ethical Committee of Copenhagen. All subjects were studied before surgery (~2 wee...

ba0001pp112 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Plasma triiodothyronine levels are positively associated with BMD and bone strength: a cross-sectional study

Moser Emil , Sikjaer Tanja , Mosekilde Leif , Rejnmark Lars

In previous studies, discrepant effects of TSH and thyroid hormones on bone have been reported. While low TSH and high thyroxine (T4) levels increase bone turnover, a recent study suggested a positive association between triiodothyronine (T3) levels and mineral deposition in bone. No data are available on bone architecture and strength in relation to thyroid status.Aim: Using a cross-sectional design, we determined whether bone dens...

ba0001pp113 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Lower bone turnover in obesity: is there a link to energy metabolism?

Viljakainen Heli , Ivaska Kaisa K , Lipsanen-Nyman Marita , Saukkonen Tero , Andersson Sture , Laitinen Kalevi , Makitie Outi

Background: Observations in rodents suggest that osteocalcin (OC) participates in glucose metabolism. Data from human studies are inconclusive and it remains unclear whether OC is simply a marker of bone turnover (BTM) or if it also mediates interactions between the skeleton and glucose homeostasis. This study determined the responses of BTMs, including OC, to oral glucose tolerance test (OGTT) in obese and normal-weight subjects.Materials and methods: P...

ba0001pp114 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Pro-angiogenic and pro-survival functions of glucose in human mesenchymal stem cells upon transplantation

Deschepper Mickael , Paquet Joseph , Manassero Mathieu , Bensidhoum Morad , Oudina Karim , Logeart-Avramoglou Delphine , Petite Herve

A major limitation in the development of cellular therapies using human mesenchymal stem cells (hMSCs) is cell survival post-transplantation. In this study, we challenged the current paradigm of hMSC survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to hMSC survival. We demonstrated that hMSCs could endure sustained near-anoxia conditions only in the presence of glucose. In this in vitro cell model, the prot...

ba0001pp115 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

On the importance of selenium for bone physiology

Pietschmann Nicole , Rijntjes Eddy , Hoeg Antonia , Schomburg Lutz

The essential micronutrient Selenium (Se) plays an important role for bone formation and homeostasis. This notion is mainly derived from animal experimental studies showing impaired bone development and reduced measures of bone quality in animals on diets with low Se supply. Selenoprotein P (SePP) functions as the central Se storage and transport protein. SePP-knockout mice have a growth deficit. SePP is taken up by a receptor-mediated mechanism. We hypothesize that impaired S...

ba0001pp116 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Effect of high doses of vitamin D on arterial properties, adiponectin, leptin, and glucose homeostasis in type 2 diabetic patients

Shargorodsky Marina

Background and aims: Vitamin D supplementation has the potential to alleviate the cardiovascular damage in diabetic patients. The present study was designed to evaluate long term impact of high doses of vitamin D on arterial properties, glucose homeostasis, adiponectin and leptin in patients with type 2 diabetes mellitus.Methods and results: In randomized, placebo-controlled study 47 diabetic patients were assigned into two groups: Group 1 received oral ...

ba0001pp117 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Relationship between bone mineral density, body composition, skin sclerosis, and serum 25(OH) vitamin D levels in systemic sclerosis

Corrado Addolorata , Neve Anna , Marucci Arcangela , Colia Ripalta , Mele Angiola , Cantatore Francesco Paolo

Introduction: Hypovitaminosis D is observed in several rheumatic autoimmune diseases, including systemic sclerosis (SSc); nevertheless, data concerning the possible determinants of reduced serum vitamin D levels in this disease are not fully investigated. The aim of this study is to evaluate the relationship between BMD, body mass composition, skin sclerosis, and serum vitamin D levels in two subsets of SSc patients.Patients and methods: 55 Post-menopaus...

ba0001pp118 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Role of estrogen replacement therapy in the control of immune system in postmenopausal osteoporosis

D'Amelio Patrizia , Sassi Francesca , Buondonno Ilaria , Fornelli Giorgia , Bonardo Elena , Isaia Giovanni Carlo

Introduction: We have recently shown that T cells play a key role in postmenopausal bone loss, here we investigate the influence of estrogen replacement therapy in the control of the immune system and osteoclastogenesis.Description of methods: We enrolled in the study 30 women with postmenopausal osteoporosis randomized to estrogen replacement therapy (HRT) or raloxifene (RLX) associated with calcium and vitamin D or calcium and vitamin D alone.<p cl...

ba0001pp119 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Vitamin D in fragile elderly women with severe osteoporosis and uncontrolled hypertension

D'Amico Ferdinando , Caronzolo Francesco , Gaglio Giovanni , Granata Antonino , Lombardo Giuseppina , Pipicella Tiziana , Spatola Pippo , Russo Enzo , Grippa Alessandro

Introduction: Aim of this study was to evaluate connections between Blood Pressure values and hypovitaminosis D states in elderly women with severe osteoporosis.Design and methods: The subjects were all >80: 63 women (mean age 84+3) affected by severe osteoporosis. In 26 women we discovered a new spinal fracture after treatment. Thirty-seven women had multiple spinal fractures (>3). Among subjects with severe osteoporosis we selected 37 ...

ba0001pp120 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Interaction between FGF23 R176W mutation and C716T nonsynonymous change (T239M, rs7955866) in FGF23 on the clinical phenotype in a family with autosomal dominant hypophosphatemic rickets

Merlotti Daniela , Rendina Domenico , Gennari Luigi , Esposito Teresa , Magliocca Sara , De Filippo Gianpaolo , Strazzullo Pasquale , Nuti Ranuccio , Gianfrancesco Fernando

Autosomal dominant hypophosphatemic rickets (ADHR) is a hereditary disorder characterized by isolate renal phosphate wasting, hypophosphatemia, and inappropriately normal 1,25(OH)2D3 levels. ADHR is caused by mutations in FGF23 protein that actively regulates phosphate homeostasis. In contrast to X-linked dominant hypophosphatemic rickets, ADHR shows incomplete penetrance, variable age at onset, and in rare cases resolution of the phosphate-wasting defect...

ba0001pp121 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Calcium dietary intake in southern lebanese women

Yaghi Yasser , Horr Fatiha El , Maan Nancy , Yaghi Kinda

Aim: The aim of this study was to assess the calcium daily food intake in Lebanese women living in South Lebanon, an under privileged area.Subjects and methods: Assessment of calcium food intake was performed with a validated questionnaire in 290 female patients attending clinics in two main referral health centers in South Lebanon.Results: The mean age of patients was 54.76 years and the mean BMI was 30.07 kg/sqm (S.D.</sma...

ba0001pp122 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Effects of long-term oral administration with methimazole on femur properties in male Wistar rats

Tatara Marcin , Golynski Marcin , Radzki Radoslaw , Bienko Marek , Krupski Witold

Methimazol is an antithyroid drug for treatment of hyperthyroidism. Methimazol inhibits the enzyme thyroperoxidase participating in thyroid hormone synthesis and reduces triiodothyronine (T3) and thyroxine (T4) blood concentrations. The study was performed to determine effects of 90-day long oral administration with methimazol on femur properties isolated from male Wistar rats. The first group (n=6) consisted of control rats. The experimental rat...

ba0001pp123 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Vitamin D supplementation during lactation: effect on maternal and offspring's vitamin D status and bone mass–double-blind randomized control trial

Czech-Kowalska Justyna , Jaworski Maciej , Latka-Grot Julita , Bulsiewicz Dorota , Pludowski Pawel , Chazan Bogdan , Pawlus Beata , Wygledowska Grazyna , Zochowska Anna , Kornacka Maria K , Kryskiewicz Edyta , Karczmarewicz Elzbieta , Dobrzanska Anna

Introduction: Optimal vitamin D intake for lactating women remains controversial. We hypothesized that 1200 IU/day (vs 400 IU/day) of vitamin D during breastfeeding will enhance maternal vitamin D status and bone mass.Methods: Healthy mothers after term, singleton delivery were randomized to receive vitamin D3: 1200 IU/day (800+400 IU/day from multivitamins) or 400 IU/day (placebo+400 IU/day from multivitamins) during lactation. Serum 25-hydroxyvitamin D...

ba0001pp124 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Polyphenols from berries of Aronia melanocarpa improve the antioxidative capacity of the bone tissue in cadmium-exposed rats

Rogalska Joanna , Brzoska Malgorzata M , Roszczenko Alicja , Galazyn-Sidorczuk Malgorzata , Jurczuk Maria

Cadmium is characterized by oxidative properties and this heavy metal-induced oxidative stress has been recognized to be involved in its injurious impact on the skeleton. Oxidative/reductive processes are an integral component of bone remodeling; however, destroying of the bone tissue oxidative/antioxidative status with excessive production and accumulation of reactive oxygen species has detrimental impact on bone metabolism. Thus, the aim of this study was to investigate whet...

ba0001pp125 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

The serum serotonin and 25-OH vitamin D levels: a study in 97 menopausal women

Carsote Mara , Popescu Mihaela , Samoila Ramona , Baloescu Rene , Muler Madalina , Gruia Adriana , Poiana Catalina

Introduction: The vitamins as B6, C, or D are involved in serotonin metabolism but mostly in central neurotransmitter pathways. There are very few clear data related to 25-OH vitamin D status and serum serotonin (SS) levels.Aim: We analyze the SS and 25-OH D.Materials and methods: We included women in menopause. The serum serotonin (SS; normal 100–400 ng/ml) and 25-OH vitamin D (normal 30–100 ng/ml) were performed in fast...

ba0001pp126 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Vitamin D: light side and best time of sunshine in Riyadh, Saudi Arabia

Alshahrani Fahad , Almalki Mussa , Aljohani Naji , Alzahrani Abdullah , Alsaleh Yossef , Holick Michel

Low levels of 25-hydroxyvitamin D have been documented among inhabitants of the wider Middle East and North African countries. Sunlight has long been recognized as a major provider of vitamin D. In this study we aimed to determine the optimum time for sun exposure in the Central region of Riyadh, Saudi Arabia. Ampoules containing 7-dehydrocholesterol in ethanol were exposed to sunlight every hour starting from sunrise until sunset in July and December. Our results demonstrated...

ba0001pp127 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Hypercalcemia following high vitamin D loading dose

Lips Paul , Neradova Aegida , van Schoor Natasja , Vervloet Mark

Vitamin D deficiency is common in older persons and non-western immigrants. Vitamin D is often started in loading doses of 50 000 IU/ml solution. Though, generally considered safe, this highly concentrated solution carries some risks as is illustrated by the following cases. A woman of >80 years old was admitted with hypercalcemia, calcium 3.27 mmol/l. She complained of nausea, thirst and polyuria for 3 months. History included cholecystitis, atrial fibrillation, myocardia...

ba0001pp128 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Mass spectrometric immunoassay for intact parathyroid hormone: correlation with immunoassay and application to clinical samples

Couchman L , Taylor D , Krystans B , Lopez M , Prakash A , Sarracino D , Garces A , Vogelsang M , Peterman S , Vadali G , Robinson S , Moniz C

Introduction: Parathyroid hormone (PTH) measurement is of use in i) differential diagnosis of hypercalcaemia and ii) patients with renal impairment at risk of bone disease. PTH immunoassays are complicated by cross-reactivity with truncated (inactive) variant forms, which accumulate in patients with renal impairment. PTH assay variability is a critical governance issue in renal medicine, suggesting an MS-based reference method is required.Aim: To develop...

ba0001pp129 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

The Ellsworth-Howard test revisited

Tang J C Y , Washbourne C J , Galitzer H , Hiemstra T , Meek C , Chipchase A , Fraser W D

Background: Pseudohypoparathyroidism (PHP) is a group of heterogeneous endocrine disorders characterised by hormone resistance, primarily to parathyroid hormone (PTH). The resistance is caused by defects in the GNAS gene, which encodes the Gsα protein that activates the cAMP pathway. PHP patients demonstrate elevated plasma PTH, hypocalcaemia, hyperphosphataemia with normal renal function. PTH resistance can be confirmed by Ellsworth-Howard test (PTH s...

ba0001pp130 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Calcium–phosphorus metabolism and calcium-regulating hormones in osteoporosis which associated with liver cirrhosis

Golovach Iryna , Mytnyk Zinovij , Vershynina Diana

Chronic liver diseases (CLD) leads to an imbalance of bone remodeling, bone mass decrease with the development of hepatic osteodystrophy, which is most often seen in osteoporosis. In the development of structural and functional deficiency of bone plays an important role of calcium–phosphorus homeostasis and coherence calcium-regulating hormones (CRH), which include parathyroid hormone (PTH) and active metabolites of vitamin D.The aim of this study w...

ba0001pp131 | Calciotropic and phosphotropic hormones and mineral metabolism | ECTS2013

Effects of zoledronic acid on hormone levels in premenopausal women with breast cancer receiving neoadjuvant or adjuvant chemotherapy and endocrine therapy: Probone II Study

Hadji Peyman , Kauka Anette , Bauer Thomas , Ziller May , Birkholz Katrin , Baier Monika , Muth Mathias , Kann Peter

Introduction: Loss in bone mineral density may occur soon after initiation of adjuvant therapy for hormone-receptor-positive (HR+), breast cancer (BC) and correlates with changes in hormone levels. Adding zoledronic acid (ZOL) to adjuvant treatment for BC can preserve/improve bone mineral density and delay disease recurrence; however, effects of ZOL on endocrine hormone levels are currently unclear.Methods: Probone II assessed the course of endocrine hor...