Searchable abstracts of presentations at key conferences on calcified tissues

ba0001oc6.4 | Mineralisation and energy metabolism | ECTS2013

Inhibition of PTH-induced vasorelaxation modulates its anabolic action

Gohin Stephanie , Chenu Chantal , Pitsillides Andrew , Arnett Timothy , Marenzana Massimo

The relationship between bone formation and blood flow is unclear. Recently, PTH was reported to activate production of nitric oxide (NO), a potent vasorelaxing agent, in endothelial cells and we and others have confirmed a strong vasorelaxing action of PTH in vivo in the mouse. Here, we tested the hypothesis that a potent NO synthase inhibitor (L-NAME: NG-nitro-L-arginine methyl ester) may alter the effect of intermittent PTH (iPTH) on b...

ba0001pp27 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Sclerostin/MEPE axis in OA: lessons from long bone development

Staines Katherine , Poulet Blandine , Farquharson Colin , Pitsillides Andrew

The re-initiation of developmental processes in osteoarthritis (OA) has emerged with similarities to endochondral ossification; responsible for long bone development. We aimed to establish the role of the Wnt inhibitor, sclerostin in endochondral ossification, and its relationship with MEPE, a calcification inhibitor with potential downstream functions. Knee joints from male Str/ort (spontaneous OA) and age-matched CBA control mice were analysed at 8, 18, and 40+ weeks of age ...

ba0001pp208 | Cell biology: osteoblasts and bone formation | ECTS2013

The positional origins of human osteoblasts dictate growth and differentiation potential and capacity for paracrine vascular cell cross-talk via VEGF

Shah Mittal , Gburcik Valentina , Sankey Andrew , Reilly Peter , Emery Roger , Clarkin Claire , Pitsillides Andrew

Successful long-term, cementless fixation of human shoulder components in osteoporotic (OP) and osteoarthritic (OA) patients poses major challenges. The possibility that enhanced osseointegration may rely on both the region of bone targeted and its relationship with the vasculature remains unexplored. We hypothesise that bone cells derived from subchondral (SC), cortical (C) and trabecular (Trb) bone regions exhibit differing osteogenic potential, which will be diminished in b...

ba0005p144 | Cell biology: osteoblasts and bone formation | ECTS2016

Primary osteoblast culture from red fox (Vulpes Vulpes)

Perpetuo Ines Pedro , Felder Alessandro , Pitsillides Andrew , Doube Michael , Orriss Isabel

Effects of whole bone scaling on isolated osteoblast behaviours are unknown. Exhibiting a huge range in size, inbred canines are an ideal species to determine such relationships. We have therefore undertaken initial studies in both male and female red foxes (Vulpes vulpes), the most abundant and accessible wild canid member in the United Kingdom.Femoral heads were removed from five fresh red fox cadavers (see details on table) and bone fragments...

ba0005p165 | Cell biology: osteoblasts and bone formation | ECTS2016

Canine osteoblasts from trabecular, cortical and subchondral bone present differences in alkaline phosphatase activity

Perpetuo Ines Pedro , Shah Mittal , Parsons Kevin , Orriss Isabel , Doube Michael , Pitsillides Andrew , Meeson Richard

Hip osteoarthritis is a cause of significant morbidity to people and their canine companions. Medical management is frequently insufficient, leading to surgery to relieve pain and regain mobility. Hip replacements are not without potential complications, including loosening and infection. Currently, there is a focus on uncemented implants to decrease these problems, however these rely on the biology of the femur for osseointegration and long-term stability. It has been previou...

ba0005p166 | Cell biology: osteoblasts and bone formation | ECTS2016

Primary osteoblast culture from domestic dog (Canis lupus familiaris)

Perpetuo Ines Pedro , Meeson Richard , Pitsillides Andrew , Doube Michael , Orriss Isabel

Effects of whole bone scaling on isolated osteoblast behaviour are unknown. With two orders of magnitude range in body mass, dog breeds are well-suited to determine such relationships.Femoral heads from three canine hip replacement surgeries were collected. Bone fragments were washed in PBS+AB/AM, trypsin-digested and incubated in 0.2% collagenase. Cells from resultant supernatant were seeded in DMEM+10% FCS+AB/AM at 37 °C, 5% CO2, g...

ba0005p208 | Cell biology: Osteocytes, mechanobiology | ECTS2016

Transgene expression by Dmp1 promoter fragments occurs in various organs

Saito Hiroaki , Taipaleenmaki Hanna , Al-Jazzar Ahmed , Gasser Andreas , Javaheri Behzad , Scudamore Cheryl , Bellido Teresita , Pitsillides Andrew A , Hesse Eric

Analysis of osteocyte function often uses promoter elements of osteocyte-specific genes i.e. SOST or Dentin-matrix-protein 1 (Dmp1) to overexpress genes or the Cre-recombinase for conditional deletion studies. However, evidence suggests that these promoters may not be osteocyte-specific, which would be critical for subsequent data interpretation. To investigate the selectivity of supposedly osteocyte-specific in vivo models, we crossed the 8kb-Dmp1-Cre mice (i) with A...