Searchable abstracts of presentations at key conferences on calcified tissues

ba0003w2.2 | Genetics of bone disease | ECTS2014


Riancho Jose

Epigenetic mechanisms regulate gene expression and are heritable through cell divisions even though they do not involve modifications in the gene sequence. They include methylation and other chemical modifications of cytosine nucleotides, histone posttranslational modifications, microRNAs and other non-coding RNAs. Most cytosines in the human genome are methylated, particularly in the inactive, tightly packed, heterochromatin of autosomes, and the inactivated X-chromosome in f...

ba0005p233 | Genetics and Epigenetics | ECTS2016

Differentially methylated regions in gene enhancers of mesenchymal stem cells from osteoporotic patients

Del Real Alvaro , Perez-Campo Flor , Sanudo Carolina , Garces Carlos , Garcia-Ibarbia Carmen , Perez-Nunez Maria I. , Riancho Jose A.

Osteoporosis (OP) is characterised by reduced bone mass, due to an insufficient osteoblast-mediated bone formation, unable to replace the bone tissue removed by osteoclasts. Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into osteoblasts, adipocytes and chondrocytes. Epigenetic marks like DNA methylation could influence the differentiation potential of these cells into osteoblasts and, consequently, the risk of OP. To explore this hypothesis, we...

ba0005p456 | Other diseases of bone and mineral metabolism | ECTS2016

Bone mineral density and TBS in patients with mutations of the alkaline phosphatase gene

Lopez-Delgado Laura , Riancho-Zarrabeitia Leyre , Valero Carmen , Tenorio Jair A , Garcia-Unzueta Mayte , Lapunzina Pablo , Riancho Jose A.

Alkaline phosphatase activity is critical for the mineralization of the bone matrix. Indeed, inactivating mutations of the ALPL gene, encoding the isoenzyme expressed in bone and liver, may result in the severe abnormalities of bone and other connective tissues that characterize hypophosphatasia. Nevertheless, the clinical spectrum of hypophosphatasia is rather broad and variable between a within families. Thus, along severe infantile forms, there are adult forms with mild man...

ba0001pp374 | Osteoporosis: pathophysiology and epidemiology | ECTS2013

Effect of two types of bariatric surgery (gastrojejunal bypass and sleeve gastroplasty) on gene expression of bone remodeling markers in Goto-Kakizaki rats

Perez-Castrillon Jose-Luis , Riancho Jose-Antonio , DeLuis Daniel , Gonzalez-Sagrado Manuel , Ruiz-Mambrilla Marta , Domingo-Anfres Maria , Conde Rosa , Primo David , Duenas-Laita Antonio

Background: Surgical treatment of type 2 diabetes, specially in obese patients, has provided good results in the control of blood glucose and Hb1Ac although its effect on bone health is not clear. The aim of this study was to evaluate gene expression of bone remodelling markers in type 2 diabetic Goto-Kakizaki (GK) non-obese rats after gastrojejunal bypass and sleeve gastroplasty, and their relationship with hormonal parameters.Materials and methods: We ...

ba0005p143 | Cell biology: osteoblasts and bone formation | ECTS2016

RUNX2, osterix and the human sclerostin gene: searching molecular and epidemiological interactions

Perez-Campo Flor , Santurtun Ana , Garcia-Ibarbia Carmen , Pascual Maria A , Valero Carmen , Garces Carlos , Sanudo Carolina , Zarrabeitia Maria T , Riancho Jose A

Sclerostin, encoded by the SOST gene, functions as an inhibitor of the Wnt pathway and thus it is an important regulator of bone homeostasis. The fact that osteoblasts, the only cells expressing SOST, lay buried deeply in the bone matrix, poses intrinsic difficulties to the study of the regulation of this gene. Since RUNX2 and SP7/OSX are two known regulators of the differentiation of cells of the osteoblastic lineage, the aim of this study was to determine t...