Searchable abstracts of presentations at key conferences on calcified tissues

ba0005p251 | Genetics and Epigenetics | ECTS2016

Establishment of an in vivo model to examine the osteoanabolic epigenome

Saito Hiroaki , Najafova Zeynab , Jahn Katharina , Taipaleenmaki Hanna , Gasser Andreas , Johnsen Steven A. , Hesse Eric

Increasing bone formation is an effective approach to prevent osteoporotic fractures. Although the intermittent administration of PTH is an established osteoanabolic therapy and an anti-Sclerostin antibody (Scle-AB) is currently being tested in phase 3 clinical trials, a great need exists for additional bone anabolic agents. Thus, in the context of a bi-national consortium we aim to uncover novel epigenomic networks controlling bone formation to identify new epigenomic approac...

ba0005cabs.op2.1 | Oral Poster Talks | ECTS2016

Antagonizing Mir-218 prevents breast cancer-induced osteolytic disease

Taipaleenmaki Hanna , van Wijnen Andre J , Stein Janet L , Hesse Eric , Stein Gary S , Lian Jane B

Signaling pathways crucial in bone development, including Wnt, are also upregulated in breast cancer cells to promote tumor growth in the skeleton, a process known as osteomimicry. Thus, we hypothesized that bone metastatic tumor cells also aberrantly express osteogenic miRNAs to support osteomimetic properties. We have previously shown that miR-218 is highly expressed in osteoblasts and promotes osteogenic differentiation. Interestingly, expression analysis revealed a signifi...

ba0005p208 | Cell biology: Osteocytes, mechanobiology | ECTS2016

Transgene expression by Dmp1 promoter fragments occurs in various organs

Saito Hiroaki , Taipaleenmaki Hanna , Al-Jazzar Ahmed , Gasser Andreas , Javaheri Behzad , Scudamore Cheryl , Bellido Teresita , Pitsillides Andrew A , Hesse Eric

Analysis of osteocyte function often uses promoter elements of osteocyte-specific genes i.e. SOST or Dentin-matrix-protein 1 (Dmp1) to overexpress genes or the Cre-recombinase for conditional deletion studies. However, evidence suggests that these promoters may not be osteocyte-specific, which would be critical for subsequent data interpretation. To investigate the selectivity of supposedly osteocyte-specific in vivo models, we crossed the 8kb-Dmp1-Cre mice (i) with A...

ba0005p253 | Genetics and Epigenetics | ECTS2016

Investigating the osteoanabolic epigenome of aging-related bone loss in humans

Ring Matthias , Saito Hiroaki , Taipaleenmaki Hanna , Najafova Zeynab , Jahn Katharina , Gasser Andreas , Haasper Carl , Gessler Roland , Gehrke Thorsten , Johnsen Steven A. , Hesse Eric

During aging bone resorption often increases while bone formation decreases, thereby reducing bone mass and bone mineral density (BMD) and leading to osteoporosis. Evidence suggests that extrinsic factors may influence bone remodeling. While poorly understood, these mechanisms may function by inducing epigenomic programs that diminish the bone forming capacity of osteoblasts. This study is part of a bi-national consortium aimed at uncovering epigenomic networks controlling the...