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Bone Abstracts (2014) 3 PP183 | DOI: 10.1530/boneabs.3.PP183

Chondrocytes and cartilage

Meniscus – Cartilage paracrine crosstalk in osteoarthritis

Stavroula Samara1, Elisavet Chatzopoulou2, Ioannis Melas2, Dimitris Messinis2, Zoe Dailiana3, Panagoula Kollia1 & Leonidas Alexopoulos2


1Department of Genetics and Biotechnology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece; 2Mechanical Engineering Department, National Technical University of Athens, Athens, Greece; 3Department of Orthopaedic Surgery, Faculty of Medicine, University of Thessalia, Larissa, Greece.

Introduction: Meniscus plays an essential role in knee joint function providing stability and load transmission. In osteoarthritis (OA), a joint disease characterized by chronic synovitis and cartilage degeneration, pathological changes in the menisci are observed. However, whether menisci contribute to the progression of OA, the underlying mechanism for meniscus-cartilage communication is still unclear. In this study we analyzed systematically the response of meniscus and cartilage explants to a number of inflammatory mediators, in order to reveal their response similarity and highlight potential crosstalks and interactions.

Methods: OA cartilage and the lateral meniscus were harvested from two patients undergoing total knee arthroplasty. Meniscus and cartilage disks (3 mm diameter) were stimulated with inflammatory mediators ((IL-1α, IL-1β, IL-12α, CSF2) (50 ng/ml), (TNF-α, IL-6, CXCL7, IL-8, CCL2, CXCL10, IFN-γ, IL-3, MIA2, IL-4) (100 ng/ml) and GROα (500 ng/ml)) for 24 h. For each condition the release of different proteins (IL-1α, IL-1β, TNF-α, IL-6, CXCL7, GROα, IL-8, CCL2, CXCL10, IFN-γ, IL-3, IL-12α, MIA-2, CSF2, IL-4) was measured in the supernatant using custom multiplexed assays on a Luminex FlexMap 3D instrument.

Results: In both tissues the major inflammatory players (IL-1α, IL-1β, TNFα) were the strongest stimuli as expected. Meniscus responses were the same up to 73 and 50% with the cartilage ones for the first and the second donors respectively. Interestingly, meniscus under certain stimuli (IL-1α, IFN-γ, CSF2, IL-8) responded differently than cartilage by releasing five different cytokines while cartilage did not.

Conclusions: Our results indicate that meniscus is affected by its inflammatory environment and responds to it as actively as cartilage. Moreover, the release of different cytokines from meniscus and cartilage suggests that meniscus can be an active player in the progression of OA. These data support the hypothesis that significant crosstalk between these two knee compartments exist and anti-inflammatory therapies should take into consideration both tissues.

Volume 3

European Calcified Tissue Society Congress 2014

Prague, Czech Republic
17 May 2014 - 20 May 2014

European Calcified Tissue Society 

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