Searchable abstracts of presentations at key conferences on calcified tissues
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43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

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Oral Communications

Catabolism and metabolism

ba0005oc4.1 | Catabolism and metabolism | ECTS2016

A small molecule inhibitor of TRAF6 dependent signaling reduces osteoclastogenesis and prevents ovariectomy induced bone loss

Marino Silvia , Bishop Ryan , Bendle Gavin , Nicolaes Gerry , Lutgens Esther , Idris Aymen

Tumour necrosis factor receptor-associated factor 6 (TRAF6) plays a key role in osteoclastogenesis through the regulation of RANK/CD40 TRAF6-mediated signaling. Mice deficient in TRAF6 exhibit high bone mass and were protected against inflammation-induced bone loss. Here we describe the effects of a small-molecule 6877002 that has been shown to prevent the binding of TRAF6 to its domain on RANK/CD40 receptor on osteoclast formation in vitro and in vivo and on...

ba0005oc4.2 | Catabolism and metabolism | ECTS2016

Inhibition of Sphingosine 1 Phosphate produced by Osteoclasts reduces chondrocyte catabolism and prevents osteoarthritis in mice

Cherifi Chahrazad , Hafsia Narjes , Latourte Augustin , Richette Pascal , Hay Eric , Cohen-Solal Martine

Purpose: High osteoclastogenesis accompanies early stages of osteoarthritis (OA). Cartilage loss is reduced when osteoclasts are inhibited in mice models with bone hyperresorption. Although several evidences show that osteoclast-produced molecules affects chondrocyte metabolism, the mechanism by which inhibition of osteoclasts protects from cartilage damage is unclear. Our purpose was to investigate the role of Sphingosine 1 Phosphate, a lipid mediator secreted by osteoclasts ...

ba0005oc4.3 | Catabolism and metabolism | ECTS2016

Connecting the dots between bone and energy metabolism: the role of Lipocalin 2

Capulli Mattia , Gemini-Piperni Sara , Maurizi Antonio , Ponzetti Marco , Teti Anna , Rucci Nadia

We previously demonstrated the involvement of Lcn2 in bone loss induced by mechanical unloading. This prompted us to investigate bone phenotype of Lcn2−/− mice by μCT, which showed an osteopenic phenotype, characterized by 40% lower trabecular bone volume, 50 and 21% lesser trabecular number and thickness, respectively, and 20% higher trabecular separation, compared to WT, while cortical thickness was significantly lower (40%) only in elderly Lcn2<s...

ba0005oc4.4 | Catabolism and metabolism | ECTS2016

Osteocyte-specific ablation of Pparγ improves energy metabolism and prevents fat accumulation but not bone loss in response to a high fat diet

Brun Julia , Ferrari Serge , Bonnet Nicolas

Pparγ is a master transcriptional regulator of energy metabolism. We demonstrated that Dmp1-Cre/Lox-mice (KO) have increased bone mass and improved energy metabolism. Here we investigated if Pparγ-deficiency in Dmp1 cells can prevent high fat diet effects on these parameters. For this purpose, WT and KO male mice aged of 16 weeks received a high fat or chow diet (HF 60% vs CD 10% of fat) for 12 weeks. Lean and fat, bone structure, metabolic rate and tissue temperatur...

ba0005oc4.5 | Catabolism and metabolism | ECTS2016

Effects of glucagon-like peptide-1 receptor agonists on bone blow flow and architecture in diabetic mice

Pereira Marie , Gohin Stephanie , Cleasby Mark , Chenu Chantal

Type 2 diabetes mellitus prevalence increases with age and the disease predisposes to increased risk of fractures and skeletal complications. One possible contributor to skeletal weakening in diabetes is a decline in blood supply. We hypothesised that bone blood flow is impaired in diabetic mice and that chronic administration of glucagon-like peptide-1 receptor agonists (GLP-1RA) can increase blood flow to bone, thereby stimulating bone formation and improving bone architectu...

ba0005oc4.6 | Catabolism and metabolism | ECTS2016

The myokine Irisin improves bone quality and strength

Colaianni Graziana , Cuscito Concetta , Mongelli Teresa , Pignataro Paolo , Liu Peng , Lu Ping , Mori Giorgio , Brunetti Giacomina , Yuen Tony , Sun Li , Reseland Janne , Colucci Silvia , Zaidi Mone , Cinti Saverio , Grano Maria

Irisin is a hormone-like myokine secreted from skeletal muscle in response to exercise. Considering that an intimate relationship between skeletal muscle and bone has been well established and that physical exercise physiologically stimulates the skeleton strengthening, we explored the involvement of Irisin on bone metabolism.Our findings demonstrated that the myokine Irisin improves cortical bone mass and geometry in vivo, supporting the idea t...