Searchable abstracts of presentations at key conferences on calcified tissues
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43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

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Oral Communications

Development and differentiation (or Aging)

ba0005oc6.1 | Development and differentiation (or Aging) | ECTS2016

Analyses of structural and functional impact of three FGFR3 mutations localized at position K650 leading to both mild and lethal dwarfism

Ebri Davide Komla , Dambroise Emilie , Benoist-Lasselin Catherine , Kaci Nabil , Barbault Florent , Legeai-Mallet Laurence

The fibroblast growth factor receptor 3 (FGFR3) activation leads to dwarfism with a spectrum of severity, hypochondroplasia (HCH), severe achondroplasia with developmental delay and acanthosis nigricans (SADDAN), and thanatophoric dysplasia (TD). Interestingly, FGFR3 mutations localized at the same position in the tyrosine kinase domain are responsible for HCH (p. Lys650Asn), SADDAN (p. Lys650Met) and TD (p. Lys650Glu).The mechanisms of FGFR3 activation ...

ba0005oc6.2 | Development and differentiation (or Aging) | ECTS2016

Bone with uncleavable type I collagen C-propeptide has abnormal development of multiple bone cell populations and increased bone mineral density with age

Barnes Aileen M , Perosky Joseph E , Blouin Stephane , Rajpar M Helen , Khoury Basma , Klaushofer Klaus , Roschger Paul , Fratzl-Zelman Nadja , Kozloff Kenneth M , Marini Joan C

Mutations in the C-propeptide cleavage site of both COL1A1 and COL1A2 cause dominant high bone mass (HBM) osteogenesis imperfecta (OI), characterized by bone hypermineralization. To elucidate the role of C-propeptide processing in bone formation, we generated heterozygous HBM mice in which both residues of the COL1A1 cleavage site were mutated to prevent cleavage by BMP1. HBM mice are smaller than WT in both weight and length and have extremely brittle bones....

ba0005oc6.3 | Development and differentiation (or Aging) | ECTS2016

The critical biomechanical role of Lipocalin 2 in the crosstalk between endothelium and osteoblasts in unloading conditions.

Veeriah Vimal , Capulli Mattia , Zanniti Angelo , Chatterjee Suvro , Rucci Nadia , Teti Anna

Angiogenesis and osteogenesis are tightly linked and dependent on each other. Lipocalin 2 (LCN2) is a mechanoresponding adipokine, strongly upregulated in osteogenic cells subjected to microgravity (0.08–0.008 g), in which it impairs osteogenesis and upregulates the osteoclastogenic cytokine, RANKL. We investigated the role of LCN2 in the crosstalk between angiogenesis and osteogenesis in simulated microgravity conditions as a model of mechanical unloading. Mouse and huma...

ba0005oc6.4 | Development and differentiation (or Aging) | ECTS2016

Extranuclear effects of estrogen on cortical bone in males is dependent on estrogen receptor A activation function-1

Farman Helen , Wu Jianyao , Gustafsson Karin , Windahl Sara , Kim Sung , Katzenellenbogen John , Ohlsson Claes , Lagerquist Marie

Estradiol (E2) signaling via estrogen receptor alpha (ERα) is important for the male skeleton as demonstrated by ERα inactivation in both mice and man. ERα mediates estrogenic effects by translocating to the nucleus and there affects gene transcription, but some effects can also be mediated via extra-nuclear actions of the receptor by, e.g., triggering cytoplasmic signaling cascades. ERα contains various domains and the role of activation functio...

ba0005oc6.5 | Development and differentiation (or Aging) | ECTS2016

Absence of cyclophilin A impairs endochondral bone formation by altering intracellular signaling pathways required for osteoblast maturation

Sargent Brandi , Ishikawa Masaki , Clevenger Charles , Yamada Yoshihiko , Cabral Wayne , Marini Joan

Using a CyPB-null mouse model, we previously demonstrated delayed folding, abnormal post-translational modification and altered crosslinking of type I collagen synthesized by osteoblasts. However, intracellular folding of collagen molecules was further delayed by CsA treatment of CyPB-null cells, suggesting involvement of additional PPIases in collagen folding. Since studies of CyPA functions in osteoblasts have not been reported, we investigated the role of this cytoplasmic P...

ba0005oc6.6 | Development and differentiation (or Aging) | ECTS2016

Loss of the longevity gene SirT1 dysregulates chondrocytes and leads to an arthritic phenotype in vivo, via impaired autophagy

Sacitharan Pradeep Kumar , Zarebska Jadwiga , Gharios George Bou , Vincent Tonia , Edwards James

Ageing is universally linked to skeletal deterioration. Common mechanisms may control both processes, where dysregulation may predispose to bone loss and osteoarthritis (OA). The epigenetic modifier SirT1 controls lifespan and decreases with age. However, the role of SirT1 in joint disease is unclear. Human tissue samples, novel genetically modified mice, a surgical disease model and advanced cellular and molecular studies were employed to explore the hypothesis that SirT1 is ...