Searchable abstracts of presentations at key conferences on calcified tissues
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43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

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Poster Presentations

Bone Matrix

ba0005p82 | Bone Matrix | ECTS2016

CRISPR/Cas9-mediated IFITM5 gene editing demonstrates that BRIL (Ser40Leu) substitution suppresses PEDF-mediated activation of PPARγ

Kang Heeseog , Marini Joan

Osteogenesis imperfecta (OI) type VI is caused by recessive null mutations in SERPINF1, encoding pigment epithelium-derived factor (PEDF), an anti-angiogenic secretory glycoprotein. Dominant mutations in IFITM5, encoding BRIL (Bone Restricted Ifitm-Like), a transmembrane protein upregulated in osteoblasts during mineralization, cause either type V OI (c.-14C>T, addition of 5 amino acids on BRIL) or atypical type VI OI (...

ba0005p83 | Bone Matrix | ECTS2016

Cortical bone matrix mineralisation is decreased in TRPV4 deficient male, but not in female mice

Roschger Paul , Misof Barbara , Fratzl-Zelman Nadja , Hoenderop Joost , Bindels Rene , van Leeuwen Johannes , Klaushofer Klaus , van der Eerden Bram

Transient receptor potential vanilloid channels (TRPVs) have been implicated in Ca2+ homeostasis and bone metabolism. In particular, TRPV4 deficiency was shown to cause sexual dimorphism in bone metabolism and osteoporotic fracture risk. Cortical bone structure was reported to be altered in male TRPV4 knock-out (TRPV4−/−) mice but not in female TRPV−/− mice compared to sex-matched wildtype (TRPV4+/+) animals.To gain knowledge on t...

ba0005p84 | Bone Matrix | ECTS2016

Anabolic parathyroid hormone (PTH) treatment does not alter periosteal bone mineral composition during primary and secondary mineralisation

Vrahnas Christina , Pearson Thomas A , Brunt Athena R , Forwood Mark R , Bambery Keith R , Tobin Mark J , John Martin T , Sims Natalie A

Parathyroid hormone (PTH) is used to stimulate bone formation in osteoporotic patients, however concerns have been raised about the quality of the matrix produced since lower levels of total matrix mineral have been reported in osteoporotic and fracture patients treated with PTH. High resolution synchrotron-based Fourier Transform Infrared Microscopy (sFTIRM) was used to determine mineral content in age-matched bone during anabolic PTH treatment, using the simplified lamellar ...

ba0005p85 | Bone Matrix | ECTS2016

MicroRNA-125b in bone matrix plays a crucial role in osteoblast-osteoclast communication

Minamizaki Tomoko , Takei Yuichiro , Nakao Yuko , Irie Yasumasa , Yoshioka Hirotaka , Tanimoto Kotaro , Kozai Katsuyuki , Yoshiko Yuji

Matrix vesicles (MVs) play a key role in bone mineralization. These nano-sized vesicles bud from apical microvilli of osteoblasts and accumulate in osteoid, and initial hydroxyapatite crystals form within these vesicles. MVs appear to share a common feature with exosomes. Recent studies have demonstrated that exosomes include microRNAs (miRNAs) as mediators of intercellular communication. We purified MVs from MC3T3-E1 cell cultures by ultracentrifugation and found approximatel...