Searchable abstracts of presentations at key conferences on calcified tissues
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43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

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Poster Presentations

Chondrocytes and cartilage

ba0005p209 | Chondrocytes and cartilage | ECTS2016

Crosstalk between FLS and chondrocytes is regulated by HIF-2α-mediated cytokines in arthritis

Ryu Je-Hwang , Koh Jeong-Tae , Hwang Yun-Chan

Rheumatoid arthritis (RA) and osteoarthritis (OA), two common types of arthritis, affect the joints mainly by targeting the synovium and cartilage. Increasing evidence indicates that a significant network connects synovitis and cartilage destruction during the progression of arthritis. We recently demonstrated that hypoxia-inducible factor (HIF)-2α causes RA and OA by regulating the expression of catabolic factors in fibroblast-like synoviocytes (FLS) or chondrocytes. To ...

ba0005p210 | Chondrocytes and cartilage | ECTS2016

Thyroid hormone locally interacts with the sympathetic nervous system to control bone linar growth

Miranda Rodrigues Manuela , Brum Patricia , de Azevedo Gouveia Cecilia Helena

It is well known that thyroid hormone (TH) is essential for normal bone growth and development. However, the mechanisms by which TH regulates these processes are poorly understood. Recently, the sympathetic nervous system (SNS) was identified as a potent regulator of bone metabolism. In vivo studies by our group have shown that TH interacts with the SNS to regulate bone mass and structure, and that this interaction involves α2 adrenoceptor (α2-A...

ba0005p212 | Chondrocytes and cartilage | ECTS2016

Histological structure of the mandibular condylar cartilage in rats of various age after 60-day intake of epichlorohydrin

Gavrilov Vladimir , Luzin Vladyslav , Kozhemyaka Ida , Mishchenko Nina

Objectives: This study is aimed at investigating of histological features of mandibular condylar cartilage (MCC) in rats after 2-month inhalation of epichlorohydrin (ECh) vapors and administration of thiotriazoline (Th) and Echinaceae tinctura (ET) as medication.Methods: The experiment involved 420 male rats (young, adult and senile). The animals were split into the groups: 1st group comprised control animals, the 2nd group comprised the animals...

ba0005p213 | Chondrocytes and cartilage | ECTS2016

The vacuolar H+ ATPase V0 subunit D2 is associated with chondrocyte hypertrophy and supports chondrocyte differentiation

Ayodele Babatunde , Mirams Michiko , Pagel Charles , Mackie Eleanor

In a recent unbiased transcriptomic study of genes associated with equine osteochondrosis, we identified several novel cartilage genes. The current study was undertaken to determine whether these genes are regulated during chondrocyte hypertrophy, and to identify novel hypertrophy-associated genes for further study in vitro. Gene expression was investigated by quantitative PCR (qPCR) in different zones of growth cartilage microdissected from equine foetal metatarsal b...

ba0005p214 | Chondrocytes and cartilage | ECTS2016

Hif1alpha leads to chondrodysplasia in MMP-deficient mice

Devignes Claire-Sophie , Duchamp de Lageneste Oriane , Gonon Alexis , Devillers Audrey , Yu Ying , Werb Zena , Provot Sylvain

Hypoxia and the hypoxia-inducible factor 1alpha (Hif1alpha) are known to play critical physiological functions in endochondral bone development. However, their role in abnormal cartilage formation (chondrodysplasia) is unknown. Our goal was to test the possibility that altered oxygen homeostasis, which would result in abnormal Hif1alpha expression and activity, could lead to chondrodysplasia. This was done using matrix metalloproteinase (MMP) 9 and 13 deficient mice, which pre...

ba0005p215 | Chondrocytes and cartilage | ECTS2016

Nutritional aspects of skeletal development

Monsonego-Ornan Efrat , Zaretsky Janna , Griess-Fishheimer Shelley , Travinsky Tamara

While the connection between under-nutrition and growth retardation is well documented, the opposite connection between over-nutrition and bone development was barely studied. For instance, obese children grow faster in height than normal-weighed children, and prospective studies demonstrated an over-presentation of obese children amongst fracture cases. Furthermore, little is known about the direct effect and the underlying cellular and molecular mechanisms of the diet or sin...

ba0005p216 | Chondrocytes and cartilage | ECTS2016

The role of CANT1 in skeletal development with a mouse model of Desbuquois dysplasia type 1

Monti Luca , Costantini Rossella , Paganini Chiara , Lecci Silvia , Maruelli Silvia , Biggiogera Marco , Cormier-Daire Valerie , Forlino Antonella , Rossi Antonio

Desbuquois dysplasia (DBQD) is a rare recessive chondrodysplasia, characterized by growth retardation, multiple dislocations and advanced carpal ossification. Two forms of DBQD have been described on the basis of the presence (type 1) or absence (type 2) of characteristic hand anomalies. DBQD type 1 is caused by mutations in the Calcium-Activated Nucleotidase 1 gene (CANT1), while DBQD type 2 is caused by mutations in the xylosiltransferase 1 gene.CANT1 ...

ba0005p217 | Chondrocytes and cartilage | ECTS2016

Effect of combined sex hormone replacement on bone/cartilage turnover in a murine model of osteoarthritis

Oh KwangJun

Background: Estrogens act on estrogen receptors distributed in articular cartilages, synovial membrane, and ligaments, which are thought to be related with degenerative changes. Meanwhile, progesterone is known to have a weak anabolic action on bone formation. This study evaluates the effects of estrogen and progesterone hormone on bone/cartilage turnover in ovariectomized (OVX) rats.Methods: Thirty-five 7-month-old female Sprague–Dawley rats were r...

ba0005p218 | Chondrocytes and cartilage | ECTS2016

Hypoxia inducible factor-1α directly induces the expression of receptor activator of nuclear factor-κB ligand in ATDC5 chondrocyte

Baek Kyunghwa , Park Hyungjung , Baek Jeong-Hwa

Reduced availability of oxygen, i.e. hypoxia, could occur during disuse, bone development, and fracture. Cartilage tissue is avascular in nature and the microenvironment of cartilage is hypoxic. Hypoxic regulation of gene expression generally involves activation of the hypoxia-inducible factor (HIF) transcription pathway. Receptor activator of nuclear factor-kappaB ligand (RANKL) is an osteoblast/stromal cell derived essential factor for osteoclastogenesis. Hypoxia-induced enh...

ba0005p219 | Chondrocytes and cartilage | ECTS2016

Characterization of COMP in zebrafish

Forte Gomes Fabiana , Koch Peter , Kobbe Birgit , Zaucke Frank , Paulsson Mats , Wagener Raimund

COMP (Cartilage Oligomeric Matrix Protein) is a member of the thrombospondin family of extracellular matrix proteins and also referred to as thrombospondin-5. Mutations in COMP lead to chondrodysplasias. Here we studied COMP in zebrafish (Danio rerio) which is a powerful vertebrate model organism. In silico analysis revealed that only one COMP coding gene exists in zebrafish and that the domain structure of COMP is fully conserved. Inspection of gene locus and phyloge...

ba0005p220 | Chondrocytes and cartilage | ECTS2016

Role of matrilin-3 in the development of osteoarthritis

Seifer Patrick , Heilig Juliane , Niehoff Anja , Paulsson Mats , Zaucke Frank

Introduction: The matrilin-3 polymorphism T298M has been described to be associated with hand osteoarthritis in the carpometacarpal joint 1 (CMC1) and with spinal disc degeneration. In vitro studies revealed that expression levels, processing and secretion of matrilin-3 T298M were similar to the wt protein. Structural analysis indicated an impact on the formation of collagen II/IX/XI heterofibrils, whereas the binding to collagen was not affected. We have now generate...

ba0005p221a | Chondrocytes and cartilage | ECTS2016

miR-214: a novel regulator of chondrogenesis?

Roberto Vania P , Conceicao Natercia , Nunes Maria J , Rodrigues Elsa , Cancela M Leonor , Tiago Daniel M

Skeletogenesis is an intricate process controlled by numerous transcriptional factors, hormones and signalling pathways. Recently, microRNAs emerged as important players in skeletogenesis but, only few were identified and most of their targets remain unknown. Previous works showed that miR-199a-2/214 cluster is essential for skeletal development and that miR-214 inhibits bone formation in mammals. However, data regarding its skeletal role in other vertebrates is scarce and its...

ba0005p221b | Chondrocytes and cartilage | ECTS2016

Vitamin D supplementation for 12 months in older people prevents bone loss and suppresses parathyroid hormone levels

Aspray Terry J , Francis Roger M , McColl Elaine , Chadwick Thomas , Stamp Elaine , Prentice Ann , Schoenmakers Inez

Background, subjects and methods: Vitamin D insufficiency in older people in the UK is common and may cause secondary hyperparathyroidism and bone loss. In a randomised, double blind intervention trial to optimise “Vitamin D status in Older People’ (VDOP) three oral dosages of vitamin D3 (12 kIU, 24 kIU or 48 kIU/month) were given for 12 months to 375 participants aged over 70 years (ANOVA) adjustment for covariables with results below presented in ascendi...