Purpose: The aim of our study is a micro-CT-based, longitudinal characterisation of ovariectomy, multideficient diet and corticoid therapy impact on the ossal microarchitecture.
Materials and methods: Four randomized groups were formed out of 32 adult female merino land sheep:
2. ovariectomized (OVX)
3. OVX+multideficient diet (OVX-D)
4. OVX-D+320 mg methylprednisolonacetat every 14 days (OVX-D-S)
Biopsies of the iliac crest were taken initially, after 3 and after 8 months. Micro-CT-based examinations of the biopsies using an isotropic voxel size of 7 μm regarding bone mineral density (BMD), relative bone volume (BV/TV), trabecular thickness (Tb.Th.), trabecular number (Tb.N.), trabecular separation (Tb.Sp.) and structural model index (SMI) were taken.
Results: The data showed no sign of osteoporosis in the control-group, in the OVX- and OVX-D group.
However, a significant change in bone mineralisation and bone architecture in the OVX-D-S group could be shown after 3 and 8 months. BMD (231.4±45.9 mg/cm3 vs 167.59±25.8 mg/cm3 vs 135.7±26.7 mg/cm3; P<0.05), BV/TV (21.2±3.8% vs 13.7±1.8% vs 12.9±2.1%; P<0.05), Tb.Th. (143±13 μm vs 101±11 μm vs 88±6 μm; P<0.05) and Tb.Sp. (581±82 μm vs 631±82 μm vs 627±91 μm; P<0.05).
With regard to Tb.N. and SMI no significant changes in the OVX-D-S group could be shown.
Conclusion: When comparing the different groups, significant changes could be found regarding BMD and microarchitecture by combining OVX, diet and methylprednisolone. This may be an interesting model for preclinical osteoporosis research, especially with regard to the development of bone substitute materials.
14 May 2016 - 17 May 2016