Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp189 | Cell biology: osteoblasts and bone formation | ECTS2013

Integrins and cadherins in mesenchymal stem cells from dental tissues: possible implication in the osteogenic differentiation process

Di Benedetto Adriana , Carbone Claudia , Oranger Angela , Brunetti Giacomina , Lorenzo Lo Muzio , Colucci Silvia , Grano Maria , Mori Giorgio

Numerous studies have reported beneficial effects of multipotent mesenchymal stem cells (MSCs) in tissue repair and regeneration. These multipotent cells can be isolated from many different adult tissues and give rise to different cell lineages. The most well-characterized source for adult stem cells is still adult bone marrow, however in the past decade, subpopulations of stem cells have been isolated from dental tissues. Dental pulp has been identified as a promising source ...

ba0003pp212 | Muscle, physical activity and bone | ECTS2014

New insights in the bone-muscle axis: the novel myokine irisin is involved in skeletal metabolism

Colaianni Graziana , Cuscito Concetta , Mongelli Teresa , Oranger Angela , Mori Giorgio , Brunetti Giacomina , Colucci Silvia , Cinti Saverio , Grano Maria

It has been recently reported that, after physical exercise activity, the skeletal muscle releases Irisin, the newly identified myokine able of driving transition of white adipocytes to brown, following a phenomenon known as the browning response. This result has suggested that skeletal muscle is crucial in the regulation of energy homeostasis, supporting the idea that it can be considered an endocrine organ that targets adipose tissue by promoting energy expenditure. In accor...

ba0005p127 | Cancer and bone: basic, translational and clinical | ECTS2016

LIGHT promotes osteolytic bone metastases in NSCLC patients

Brunetti Giacomina , Belisario Dimas Carolina , Alliod Valentina , Buffoni Lucio , Colucci Silvia , Grano Maria , Ferracini Riccardo , Roato Ilaria

LIGHT is a TNF superfamily member, expressed by activated T cells. It is involved in erosive bone disease, such as rheumatoid arthritis where it stimulates osteoclastogenesis. In multiple myeloma, LIGHT promotes osteolysis by increasing osteoclastogenesis and inhibiting osteoblastogenesis. We investigated whether LIGHT has a role in the osteolytic bone metastatic process induced by non small cell lung cancer (NSCLC), which is a tumor with a marked osteotropism. We analysed by ...

ba0001pp503 | Paediatric bone disease | ECTS2013

High dickkopf-1 levels in sera and leukocytes from children with 21-hydroxylase deficiency on chronic glucocorticoid treatment

Brunetti Giacomina , Maria Felicia Faienza , Piacente Laura , Ventura Annamaria , Oranger Angela , Carbone Claudia , Benedetto Adriana Di , Colaianni Graziana , Mori Giorgio , Colucci Silvia , Cavallo Luciano , Grano Maria

Children with 21-hydroxylase deficiency (21-OHD) need chronic glucocorticoid (cGC) therapy to replace congenital deficit of cortisol synthesis, and this therapy is the most frequent and severe form of drug-induced osteoporosis. In the study we enrolled 18 patients (9 females) and 18 sex- and age-matched controls. We found in 21-OHD patients high serum and leukocyte levels of dickkopf-1 (DKK1), a secreted antagonist of the Wnt/β-catenin signaling pathway, known to be a key...

ba0002op2 | (1) | ICCBH2013

High FSH serum levels may support the altered bone remodeling in Turner syndrome patients

Brunetti Giacomina , Ventura Anna Maria , Piacente Laura , Oranger Angela , Ciccarelli Maria , Mori Giorgio , Colucci Silvia , Cavallo Luciano , Grano Maria , Faienza Maria Felicia

Objective: Turner syndrome (TS) is a chromosomal aberration characterized by total or partial loss of one of the two X-chromosomes, and affects about 1 in every 2500 girls. TS patients can develop the bone disease with decreased bone density and selective reduction in cortical bone thickness, which probably contributes to the increased fracture risk. However, the mechanisms underlying the bone disease remain poorly understood. Thus, the aim of this study was to investigate the...

ba0003pp152 | Cell biology: osteoclasts and bone resorption | ECTS2014

Involvement of LIGHT in multiple myeloma bone disease

Oranger Angela , Brunetti Giacomina , Mori Giorgio , Carbone Claudia , Gigante Isabella , Mongelli Teresa , Taurino Grazia , Rizzi Rita , Mestice Anna , Zallone Alberta , Specchia Giorgina , Colucci Silvia , Grano Maria

Multiple myeloma (MM)-bone disease occurs in 70 to 80% of patients at MM diagnosis, and up to 90% at relapse; skeletal related events cause high morbidity and mortality. MM-bone disease consists of lytic lesions arising as a consequence of an unbalanced bone remodelling due to osteoclast (OC) activation, and osteoblast inactivation. Osteoclastogenesis may be under immune cell regulation through the production of numerous cytokines, such as LIGHT/TNFSF14, a newly identified mem...

ba0005oc4.6 | Catabolism and metabolism | ECTS2016

The myokine Irisin improves bone quality and strength

Colaianni Graziana , Cuscito Concetta , Mongelli Teresa , Pignataro Paolo , Liu Peng , Lu Ping , Mori Giorgio , Brunetti Giacomina , Yuen Tony , Sun Li , Reseland Janne , Colucci Silvia , Zaidi Mone , Cinti Saverio , Grano Maria

Irisin is a hormone-like myokine secreted from skeletal muscle in response to exercise. Considering that an intimate relationship between skeletal muscle and bone has been well established and that physical exercise physiologically stimulates the skeleton strengthening, we explored the involvement of Irisin on bone metabolism.Our findings demonstrated that the myokine Irisin improves cortical bone mass and geometry in vivo, supporting the idea t...

ba0005p203 | Cell biology: osteoclasts and bone resorption | ECTS2016

The role of light (TNFSF14) on bone remodeling

Brunetti Giacomina , Gigante Isabella , Oranger Angela , Colaianni Graziana , Di Benedetto Adriana , Tamma Roberto , Lippo Luciana , Pignataro Paolo , Di Comite Mariasevera , Mori Giorgio , Tamada Koji , Ware Carl , Colucci Silvia , Grano Maria

LIGHT (TNFSF14), expressed by different cells of the immune system, binds two trans-membrane receptors: HVEM and LTβR. It is over-expressed in erosive rheumatoid arthritis and lytic myeloma-bone disease and controversial data have been published on its role osteoclast (OC) formation in vitro. Here, we investigated the role of LIGHT on in vitro murine osteoclastogenesis model and bone phenotype in LIGHT−/− mice. Firstly, we showed that murin...