Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp16 | Bone biomechanics and quality | ECTS2014

Inhibition of RANKL-mediated bone remodeling decreases bone damage and improves strength in response to fatigue loading

Bonnet Nicolas , Gerbaix Maude , Kostenuik Paul , Ominsky Mike , Ferrari Serge

Antiresorptives consistently improve bone mass and structural strength in normally- and under-loaded bones, but concerns have been raised regarding potential effects on skeletal adaptation to fatigue loading, including damage accumulation and atypical fractures. We thus inhibited or activated osteoclasts with OPG-Fc or RANKL treatment, respectively, and evaluated bone damage and strength after fatigue in the early and later phases of repair. Adult male mice were treated with R...

ba0001pp432 | Osteoporosis: treatment | ECTS2013

Effects of a mutated sclerostin peptide on bone and lean mass in mice

Gerbaix Maude , Pierroz Dominique , Bonnet Nicolas , Boschert Verena , Mueller Thomas , Ferrari Serge

Sclerostin, a product of osteocytes, is known to inhibit Wnt signaling by binding the LRP5/6 receptor.We investigated the effects of a mutated mouse sclerostin protein (muScl, R118A/R144A) with potential sclerostin antagonistic activity. In vitro, muScl fully competed with wild type sclerostin for binding to LRP6, whereas its IC50 for Wnt3a activity was 4× higher than sclerostin (i.e. 600 nM). Moreover, serum osteocalcin increased in mice a...

ba0005p31 | Bone biomechanics and quality | ECTS2016

Site specific bone loss induced by spaceflight in mice: a multiscale evaluation of trabecular and cortical structure and quality

Gerbaix Maude , Gnyubkin Vasily , Delphine Farlay , Helene Follet , Patrick Ammann , Cecile Olivier , Francoise Peyrin , Norbert Laroche , Boris Shenkman , Guillemette Gauquelin-Koch , Laurence Vico

Intrinsic quality, micro and nano structure of bone after 1 month of spaceflight and during 1 week of recovery had never been investigated. The aim was to explore these properties in appendicular and axial skeleton of mature mice.Ten C57/Bl6 male mice flew on the 30-day space Russian BION-M1 high-orbit satellite mission (Biomedical Ethics Commission of IBMP, n°319). Five were euthanized 12 h after landing, the others 8 days later. A ground control g...