Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp98 | Bone development/growth and fracture repair | ECTS2013

Identification and characterization of a mesenchymal progenitor cell population involved in fracture healing

Matthews Brya , Grcevic Danka , Wang Liping , Hagiwara Yusuke , Adams Douglas , Kalajzic Ivo

Fracture healing is a multistep process that involves many cell lineages and is still not fully understood. We aimed to identify and characterize population of mesenchymal progenitor cells during its commitment within a fracture callus. To identify and trace cells in periosteum and bone marrow we used αSMA promoter-driven inducible Cre expression (αSMA-CreERT2) combined with a Cre-activated tdTomato reporter (Ai9) to generate αSMACre/Ai9 mice. Tibias, fixed with...

ba0003pp4 | Arthritis and other joint diseases: translational and clinical | ECTS2014

Phenotypic and functional characterization of osteoclast progenitors in circulatory and synovial compartments of patients with rheumatoid arthritis

Sucur Alana , Jajic Zrinka , Ikic Marina , Ivcevic Sanja , Kelava Tomislav , Grcevic Danka

Rheumatoid arthritis (RA) is marked by the persistent inflammation and osteodestruction, causing progressive disability. Osteoclast progenitors (OCPs) represent a subpopulation of peripheral blood monocytes and have been shown to be present in healthy subjects and arthritic patients. The aim of our study was to determine the phenotype, frequency and osteoclastogenic potential of OCPs in the peripheral blood and synovial fluid of RA patients in response to anti-TNF therapy....

ba0005p11 | Arthritis and other joint diseases: translational and clinical | ECTS2016

Chemotactic signals mediating osteoclast progenitor migration in collagen induced arthritis

Flegar Darja , Sucur Alan , Markotic Antonio , Kelava Tomislav , Kovacic Natasa , Ivcevic Sanja , Zrinski-Petrovic Katerina , Katavic Vedran , Grcevic Danka

Introduction: Collagen induced arthritis (CIA) is a mouse model of rheumatoid arthritis marked by persistent inflammation and enhanced osteoresorption at the affected joints as well as systemic osteopenia due to increased activation of osteoclast progenitors (OCP). Abundantly secreted chemokines presumably attract OCPs to the inflamed sites. Our goal was to identify chemotactic signals important for OCPs recruitment in CIA.Methods: After obtaining approv...

ba0001pp174 | Cell biology: osteoblasts and bone formation | ECTS2013

Elevated levels of serotonin decrease bone volume by direct effects on bone turnover in rats

Erjavec Igor , Bordukalo-Niksic Tatjana , Brkljacic Jelena , Pauk Martina , Grgurevic Lovorka , Thompson David D , Paralkar Vishwas M , Cicin-Sain Lipa , Vukicevic Slobodan , Mokrovic Gordana , Kesic Maja , Grcevic Danka

Elevated levels of circulating serotonin have been reported to decrease bone mineral density1. Conversely, reduced serotonin (5HT) in mice lacking TPH1, the rate limiting enzyme for 5HT synthesis, was reported to be anabolic to the skeleton with high osteoblastic activity2. However, in other studies TPH1 deletion led to either an initial increase in BMD due to inhibition of osteoclastic bone resorption3, or had no bone effect4</su...

ba0003pp68 | Bone development/growth and fracture repair | ECTS2014

Increased expression of PTX3 in non-hematopoietic periosteal cells during fracture healing

Kelava Tomislav , Ivcevic Sanja , Katavic Vedran , Kovacic Natasa , Cvija Hrvoje , Petrovic Katerina Zrinski , Kuzmac Sania , Kalajzic Ivo , Bottazzi Barbara , Grcevic Danka

Pentraxin 3 (PTX3) is a highly conserved member of the long pentraxins subfamily produced in response to proinflammatory stimuli. Its biological roles have been associated to infection, female fertility and angiogenesis. We aimed to elucidate the role of PTX3 in the process of fracture healing.WT mice and mice deficient for the PTX3 gene (PTX3-KO) were used, after obtaining the approval from the Ethical committee. Cells from bone marrow and endosteal/per...

ba0005p10 | Arthritis and other joint diseases: translational and clinical | ECTS2016

Identifying cell populations coupling inflammation to osteoresorption in arthritis

Mosler Elvira Lazic , Flegar Darja , Sucur Alan , Fadljevic Martina , Kelava Tomislav , Markotic Antonio , Ivcevic Sanja , Zrinski-Petrovic Katerina , Katavic Vedran , Grcevic Danka , Kovacic Natasa

Rheumatoid arthritis (RA) is characterised by periarticular bone destruction leading to permanent disability. Some other forms of arthritis, such as arthritis in systemic lupus erythematosus (SLE), rarely produce bone lesions. Corresponding to that, inactivation of Fas produces a murine SLE-like phenotype, but alleviates bone loss during antigen-induced arthritis (AIA). Aim of this study was to identify cell populations differentially regulated in non-resorptive vs. resorptive...

ba0003pp77 | Bone development/growth and fracture repair | ECTS2014

Elevated blood serotonin decreases bone volume via a mechanism involving insulin and calcitriol

Erjavec Igor , Bordukalo-Niksic Tatjana , Brkljacic Jelena , Grcevic Danka , Pauk Martina , Grgurevic Lovorka , Mokrovic Gordana , Kesic Maja , Durdevic Dragan , Rogic Dunja , Thompson David D , Paralkar Vishwas M , Cicin-Sain Lipa , Vukicevic Slobodan

To further characterize the role of serotonin (5HT) in bone metabolism, Wistar-Zagreb rat model consisting of the high-5HT and low-5HT subline with different platelet 5HT content and transporter (5HTT) activity has been used.Primary osteoclasts from the high-5HT subline had an increased expression of 5HTT, 5HT–2A receptor, Trap and cathepsin K, while osteoblasts showed no difference in the expression of 5HT elements or osteoblastic markers. However,...