Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp98 | Bone development/growth and fracture repair | ECTS2013

Identification and characterization of a mesenchymal progenitor cell population involved in fracture healing

Matthews Brya , Grcevic Danka , Wang Liping , Hagiwara Yusuke , Adams Douglas , Kalajzic Ivo

Fracture healing is a multistep process that involves many cell lineages and is still not fully understood. We aimed to identify and characterize population of mesenchymal progenitor cells during its commitment within a fracture callus. To identify and trace cells in periosteum and bone marrow we used αSMA promoter-driven inducible Cre expression (αSMA-CreERT2) combined with a Cre-activated tdTomato reporter (Ai9) to generate αSMACre/Ai9 mice. Tibias, fixed with...

ba0007p133 | (1) | ICCBH2019

Losartan reduces circulating TGFb and CTX and increases vertebral bone mass in the OIM mouse

Bishop Nick , Kalajzic Ivo , Arshad Fawaz , Lefley Diane , Gossiel Fatma , Ottewell Penny

Objectives: Losartan is an angiotensin II receptor type 1 (AT1) antagonist. Losartan reduces circulating TGFb concentrations in a variety of myopathic models. We hypothesised that losartan administration to the murine osteogenesis imperfecta model OIM would result in lower circulating TGFb and CTX (bone resorption marker) and increase bone mass.Methods: All procedures were approved by UConn Health Institutional Animal Care and Use Committee and performed...

ba0003pp68 | Bone development/growth and fracture repair | ECTS2014

Increased expression of PTX3 in non-hematopoietic periosteal cells during fracture healing

Kelava Tomislav , Ivcevic Sanja , Katavic Vedran , Kovacic Natasa , Cvija Hrvoje , Petrovic Katerina Zrinski , Kuzmac Sania , Kalajzic Ivo , Bottazzi Barbara , Grcevic Danka

Pentraxin 3 (PTX3) is a highly conserved member of the long pentraxins subfamily produced in response to proinflammatory stimuli. Its biological roles have been associated to infection, female fertility and angiogenesis. We aimed to elucidate the role of PTX3 in the process of fracture healing.WT mice and mice deficient for the PTX3 gene (PTX3-KO) were used, after obtaining the approval from the Ethical committee. Cells from bone marrow and endosteal/per...