Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp33 | Bone biomechanics and quality | ECTS2013

Influence of the organic matrix of mineralized tissues on their dynamic mechanical properties assessed by scanning acoustic microscopy

Blouin Stephane , Puchegger Stephan , Klaushofer Klaus , Roschger Paul , Fratzl Peter

Mineralized tissues like bone, articular calcified cartilage or mineralized turkey leg tendon (MTLT) are build by a composite of hydroxyapatite nano-particles and organic matrix. In bone and MTLT the matrix is formed by collagen type-I, but in contrast to bone in MTLT the collagen is uniaxial orientated, while in cartilage the matrix consists of collagen type-II and proteoglycans.Composition/orientation differences were investigated by a new scanning aco...

ba0003pp19 | Bone biomechanics and quality | ECTS2014

Scanning acoustic microscopy reveals heterogeneity of mechanical properties due to collagen orientation in mice cortical bone

Blouin Stephane , Puchegger Stephan , Klaushofer Klaus , Roschger Paul , Fratzl Peter

The local mechanical properties of bone are influenced not only by the material chemical composition but also by the spatial arrangement of the component’s e.g. orientation of collagen matrix. However, not much is known about local elastic modulus variations in cortical bone. Our goal was to use acoustic imaging to map elastic properties of murine bone with a several microns resolution. Rodent long bones exhibit a permanent growth with endosteal/periosteal bone formation ...

ba0003pp22 | Bone biomechanics and quality | ECTS2014

Bone matrix mineralization is preserved during perimenopausal stage in healthy women

Misof Barbara , Roschger Paul , Recker Robert , Klaushofer Klaus

Menopause is accompanied by a decrease in bone mineral density which can be caused by a reduction in bone volume and/or degree of bone matrix mineralization. Both of them are suggested to contribute to the increased fracture risk in postmenopausal individuals. In the present work, we aimed for information whether a drop in bone matrix mineralization is occurring in the perimenopausal stage of women. For this purpose, we measured the bone mineralization density distribution (BM...

ba0004oc4 | (1) | ICCBH2015

Increased bone matrix mineralization in treatment-naive children with Crohn's disease

Roschger Paul , Misof Barbara , Klaushofer Klaus , Rauch Frank , Ward Leanne

Background: Crohn’s disease (CD) is an inflammatory bowel disease which affects many organ systems including the skeleton. In children with CD, bone mineral density is frequently low. Bone metabolic abnormalities, including lower biochemical measures of bone turnover (NTX) as well as decreased bone formation indices at the tissue level have been reported. The aim of our study was to gain information on the bone matrix mineralization in CD.Methods: W...

ba0001pp132 | Cancer and bone: basic, translational and clinical | ECTS2013

The central role of the histone demethylase JHDM1D in the regulation of tumor associated genes in bone tumor-related cells

Thaler Roman , Spitzer Silvia , Haider Florian , Karlic Heidrun , Klaushofer Klaus , Varga Franz

Tumor development occurs often by over-activation of members of the RAS-oncogene family (small GTPases (sGTPs)). By blocking the mevalonate pathway, aminobisphosphonates (BPs), and statines prevent activation of GTPs by inhibiting their post-translational prenylation. As we have shown, this induces apoptosis in U2OS osteosarcoma cells by re-activation of FAS expression via epigenetic DNA demethylation (1).The histone demethylase JHD...

ba0001pp345 | Osteoporosis: pathophysiology and epidemiology | ECTS2013

Mortality after osteoporosis-related hip fractures in Austria 2008–2010

Brozek Wolfgang , Reichardt Berthold , Kimberger Oliver , Kritsch Daniela , Klaushofer Klaus , Zwettler Elisabeth

Osteoporosis-related hip fractures represent a substantial cause of mortality and morbidity in industrialized countries; nonetheless, past studies in Austria lack mortality figures save during hospitalization (in-hospital mortality).We therefore retrospectively retrieved pseudonymized invoice data from Austrian social insurance authorities covering roughly 98% of the entire population including 31 548 subjects over 51 years of age who sustained first hip...

ba0001pp392 | Osteoporosis: treatment | ECTS2013

Anti-osteoporosis treatment amongst austrian hip fracture patients: status quo, and effects on mortality and subsequent fracture risk

Brozek Wolfgang , Reichardt Berthold , Kimberger Oliver , Kritsch Daniela , Klaushofer Klaus , Zwettler Elisabeth

Osteoporosis is commonly known as the prime risk factor for hip fracture in the elderly. We thus evaluated status and effect of osteoporosis treatment amongst hip fracture patients in a large Austrian cohort.Retrospectively retrieved pseudonymized invoice data from Austrian social insurance authorities covering roughly 98% of the entire population included 31 548 subjects over 50 years with first hip fractures between July 2008 and December 2010, with fo...

ba0001pp456 | Other diseases of bone and mineral metabolism | ECTS2013

Altered bone material properties in HLA-B27 rats, an animal model for arthritis, ankylosing spondylitis, and gastrointestinal inflammation

Gamsjaeger Sonja , Paschalis Eleftherios P. , Zoehrer Ruth , Klaushofer Klaus , Tatakis Dimitris N.

HLA-B27 transgenic rats, developed by inserting into rat genome the gene for HLA-B27, a human Class I major histocompatibility molecule involved in antigen presentation, spontaneously develop arthritis, ankylosing spondylitis, gastrointestinal inflammation, and severe alveolar bone loss, among other signs of a generalized inflammatory response. Clinical manifestations in these rats closely resemble features of HLA-B27–associated diseases in humans. More recently, investig...

ba0003oc1.3 | Phosphate metabolism, fracture repair and osteoarthritis | ECTS2014

Klotho deficiency impairs mineralization by vitamin D hormone-driven upregulation of osteopontin and pyrophosphate in bone

Murali Sathish Kumar , Roschger Paul , Zeitz Ute , Klaushofer Klaus , Andrukhova Olena , Erben Reinhold G.

Lack of Klotho, the co-receptor for the bone-derived hormone fibroblast growth factor-23 (FGF23), leads to a severe impairment in bone mineralization in mice. However, the mechanisms underlying the Klotho deficiency-associated defects in bone mineralization are still poorly understood. Here, we sought to elucidate the vitamin D independent role of Klotho in bone mineralization. To ablate increased vitamin D signaling in Klotho knockout mice (Kl−/−), we c...