Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp87 | Bone development/growth and fracture repair | ECTS2013

Premixed calcium phosphate cement as a carrier for bone morphogenetic protein 2

Walters Nick , Hulsart-Billstrom Gry , Engqvist Hakan , Larsson Sune

Introduction: A BMP-2-containing premixed calcium phosphate cement (PCPC) could potentially provide the surgeon with an easy-to-handle alloplastic material with osteoinductive properties, accelerating bone ingrowth by stimulating osteogenic differentiation.Methods: The release profile of BMP-2 at 0.05 and 0.50 mg/ml from the PCPC was characterized over a period of 1 week using ELISA.Results: The PCPC released BMP-2 in a biphasic ma...

ba0001pp85 | Bone development/growth and fracture repair | ECTS2013

The retention and bioactivity of rhBMP-2 released from a bisphosphonate-linked hyaluronan-based hydrogel

Hulsart-Billstrom Gry , Yuen Pik-Kwan , Marsell Richard , Hilborn Jons , Larsson Sune , Ossipov Dmitri

Introduction: There are several disadvantages with the present carriers used in rhBMP-2 products including risk for immunological response, inefficient release and poor handling properties. Our aim was to examine the release of rhBMP-2 from a bisphosphonate-linked hyaluronan hydrogel with the hypothesis that it would cause a slower release.Methods: Triplicates of hydrogels with rhBMP-2 and with (HA-BP) or without (HA) bisphosphonate were prepared, after ...

ba0001pp86 | Bone development/growth and fracture repair | ECTS2013

The effect of incubation time of preformed injectable hydrogels on bone formation when used as carrier of rhBMP-2

Piskounova Sonya , Hulsart-Billstrom Gry , Gedda Lars , Bergman Kristoffer , Hilborn Jons , Larsson Sune , Bowden Tim

Introduction: Hydrogels has demonstrated efficacy as carriers for growth factors. Our aim was to investigate the effect of curing-time of modified hyaluronan on bone formation.Methods: Hydrogels with rhBMP-2 were cross-linked for 14 and 3 days, 5 h or 1 min before injection. Preformed gels were injected s.c. in 5 rats, the rats were killed after 5 weeks. Explanted samples were radiographed and scanned by pQCT.Results: Bone formatio...

ba0005p76 | Bone development/growth and fracture repair | ECTS2016

Multicentre study reveals poor correlation between in vitro and in vivo assessments of biomaterials for bone-regeneration

Billstrom Gry Hulsart , Dawson Jon , Hofmann Sandra , Muller Ralph , Stoddart Martin , Alini Mauro , Redl Heinz , Haj Alicia El , Brown Robert , Salih Vehid , Hilborn Jons , Larsson Sune , Oreffo Richard

Introduction: Research on biomaterials for bone regeneration generates a plethora of new biomaterials requiring evaluation with reliable and comparable methods of biocompatibility and functionality for clinical translation. To reduce the burden of in vivo assessment, there is a need for refined in vitro assays that are predictive of in vivo outcomes. This retrospective study correlated in vitro results with in vivo outcomes observed...