Searchable abstracts of presentations at key conferences on calcified tissues
Previous issue | Volume 5 | ECTS2016 | Next issue

43rd Annual European Calcified Tissue Society Congress

Rome, Italy
14 May 2016 - 17 May 2016

Card image cap

Poster Presentations

Bone development/growth and fracture repair

ba0005p43 | Bone development/growth and fracture repair | ECTS2016

Serum of patients with active rheumatoid arthritis inhibits differentiation of osteochondrogenic precursor cells

Pathak Janak L , Verschueren Patrick , Lems Willem F , Bravenboer Nathalie , Klein-Nulend Jenneke , Bakker Astrid D , Luyten Frank P

Delayed fracture healing is frequently experienced in patients with systemic inflammation such as during rheumatoid arthritis (RA). The reasons for this are diverse, but could also be caused by inflammatory cytokines and/or growth factors in serum from patients with active disease. We hypothesized that serum from patients with active RA contains circulating inflammatory factors that inhibit differentiation of osteochondrogenic precursors.Serum was obtain...

ba0005p44 | Bone development/growth and fracture repair | ECTS2016

Effect of a mixture of GOS/FOS® on calcium (Ca) and phosphorus (P) absorption and bone retention in ovariectomized osteopenic rats fed a low calcium diet

Bryk Gabriel , Coronel Magali Zeni , de Portela Maria Luz Pita Martin , Zeni Susana Noemi

Dietary Ca insufficiency is a common finding, independently of socio-economic status. A low Ca intake (CaI) increases bone turnover leading to bone loss. This effect becomes more important during estrogen withdrawing. We previously found that a mixture of galactooligosaccharides (GOS) and fructooligosaccharides (FOS) enhances Ca and P absorption (Abs), being a suitable tool to optimize its bioavailability and consequently bone health. The present study evaluated the effect of ...

ba0005p45 | Bone development/growth and fracture repair | ECTS2016

Mast cells regulate inflammation and bone regeneration in fracture healing

Kroner Jochen , Kovtun Anna , Messmann Joanna , Strauss Gudrun , Seitz Sebastian , Schinke Thorsten , Dudeck Anne , Ignatius Anita

Mast cells (MCs) are pro-inflammatory sensor and effector cells of the immune system. MCs seem to play a role in bone metabolism, because patients with systemic mastocytosis develop osteoporosis. MCs are present in the fracture callus during bone healing, however, their function has not yet been investigated. Here, we examined the role of MCs in the inflammatory and repair phase during fracture healing.Male 12-week old MC deficient mice (Mcpt5-Cre R-DTA)...

ba0005p46 | Bone development/growth and fracture repair | ECTS2016

Bone regeneration using transcript-activated matrices for sustained messenger RNA delivery

Badieyan Zohreh Sadat , Aneja Manish , Berezhanskyy Taras , Rudolph Carsten , Plank Christian

Transcript therapies, using chemically modified messenger RNAs (cmRNAs), are emerging as safer yet promising substitutes for gene and recombinant protein therapies. However, their applications have been limited due to transient translation and relative low stability of cmRNAs, compared to DNAs. Here we showed that vacuum-dried cmRNA-loaded collagen sponges, so called Transcript-Activated Matrices (TAMs), could serve as depots for sustained cmRNA delivery, providing steady stat...

ba0005p47 | Bone development/growth and fracture repair | ECTS2016

Bone-forming ability of recombinant human bone morphogenetic protein 2 after repeated administration in mice

Koh Jeong-Tae , Kim Yuri , Son Hye-Ju , Hwang Yun-Chan , Ryu Je-Hwang

Delivery of recombinant human bone morphogenetic protein (rhBMP2) with various carriers has been showing the successful induction of bone formation in many bony defects, including oral and maxillofacial regions. However, effectiveness of the exogenous proteins, when repeatedly administered into different regions in an individual, has not been determined. The present study was aimed to examine alterations of ectopic or orthotopic bone generation and serum level of anti-BMP2 ant...

ba0005p48 | Bone development/growth and fracture repair | ECTS2016

Biocompatibility of hydroxyapatite derived from whitemouth croaker (Micropogonias furnieri)

Ribeiro Daniel , Yamamura Hirochi , Silva Victor , Ussui Valter , Lazar Dolores , Rosso Veridiana , Renno Ana Claudia

The aim of this study was to investigate the biocompatibility of hydroxyapatite (HAP) powder from whitemouth croaker fish (Micropogonias furnieri). For this purpose, fragments from HAP with 0.5 cm2 were inserted in the subcutaneous tissue of animals. After 7, 15, and 30 days, histopathological analysis was performed. The results showed that it was possible to detect tissue reactions closely related to cytotoxicity in a time-exposure manner. At day 7, modera...

ba0005p49 | Bone development/growth and fracture repair | ECTS2016

The effects of combined teriparatide and denosumab on bone regeneration

Kitaguchi Kazuma , Kashii Masafumi , Sugiura Tsuyoshi , Tokimitsu Morimoto , Noguchi Takaaki , Makino Takahiro , Ebina Kosuke , Kaito Takashi , Yoshikawa Hideki

Objective: The purpose of this study is to investigate the effects of the combined teriparatide (TPTD) and denosumab (DMAB) on both cortical and cancellous bone regeneration.Materials and methods: Bone defects were created in the diaphysis of the right femur and in the epimetaphysis of the left femur in 8-week old female C57/BL6N mice. After making bone defects, Mice were given either saline or 40 μg/kg TPTD for 20 days (5× per week) and were s...

ba0005p50 | Bone development/growth and fracture repair | ECTS2016

Prostaglandin IP agonist promotes osteoblastic differentiation and BMP induced bone formation

Kanayama Sadaaki , Kaito Takashi , Kashii Masafumi , Makino Takahiro , Morimoto Tokimitsu , Kitaguchi Kazuma , Ishiguro Hiroyuki , Yoshikawa Hideki

Introduction: A synthetic prostacyclin IP receptor agonist (ONO-1301) has been reported to induce the production of endogenous HGF and VEGF in fibroblasts by stimulating cAMP production.Materials and methods: In vitro; murine primary osteoblasts and cell line (ST2, MC3T3-E1, C2C12) were treated with BMP-2 (0–100 ng/ml) and ONO-1301 (0–10−6 M). ALP assay and cell proliferation assay were performed. In vivo analysis, Col...

ba0005p51 | Bone development/growth and fracture repair | ECTS2016

Rictor plays a critical role in bone mass and strength with the involvement of mtorc2 pathway in osteoblasts

Liu Dong-mei , Zhao Lin , Liu Ting-ting , Jiao Pei-lin , Zhao Dian-dian , Shi Mei-Shu , Tao Bei , Sun Li-hao , Zhao Hong-yan , Liu Jian-min

Mammalian target of rapamycin (mTOR) functions mainly in the form of two complexes, namely mTORC1 and mTORC2, which are distinct in their unique components, raptor and rictor. Here, we focused on bone phenotypes in mice with a specific deletion of rictor using a Cre recombinase gene whose expression was driven by the promoter of osteocalcin. All procedures involving mice were approved by the Institutional Animal Care and Use Committee of the local admin. DXA analysis showed a ...

ba0005p52 | Bone development/growth and fracture repair | ECTS2016

Effect of intermittent administration of teriparatide (PTH 1-34) on BMP induced bone regeneration in a rat critical-sized femoral defect model

Kanayama Sadaaki , Kaito Takashi , Kashii Masafumi , Makino Takahiro , Morimoto Tokimitsu , Kitaguchi Kazuma , Ishiguro Hiroyuki , Yoshikawa Hideki

Introduction: Indirect inhibition of the extracellular BMP antagonist network and the activation of Wnt signaling by PTH1-34 suggest the possibility of synergistic effect on bone regeneration by the co-administration of BMP and PTH1-34.Materials and methods: In a rat critical-sized femoral defect (7 mm) model, SD rats (n=32) were operated by absorbable collagen sponge containing two different BMP-2 dosage treatments; 1) 2 μg (low dose) and ...

ba0005p53 | Bone development/growth and fracture repair | ECTS2016

Effect of bioactive glass-ceramic scaffold associated with bone marrow - or adipose-derived mesenchymal stem cells on bone formation under osteoporotic conditions

Freitas Gileade , Lopes Helena , Almeida Adriana , de Souza Luiz , Siessere Selma , Regalo Simone , Beloti Marcio , Rosa Adalberto

In this study, we evaluated the effect of the association of a bioactive glass-ceramic scaffold (Biosca) with mesenchymal stem cells derived from either bone marrow (BM-MSCs) or adipose tissue (AT-MSCs) on bone formation in calvarial defects of osteoporotic rats. Wistar rats were submitted to bilateral ovariectomy (OVX) or only to the surgical stress (Sham), under approval of the Committee of Ethics in Animal Research. After 5 months, 5-mm unilateral calvarial defect was creat...

ba0005p54 | Bone development/growth and fracture repair | ECTS2016

Role of interleukin-6 in the early and late fracture healing phase

Prystaz Katja , Kovtun Anna , Kaiser Kathrin , Heidler Verena , Kroner Jochen , Haffner-Luntzer Melanie , Ignatius Anita

Interleukin-6 (IL-6) plays an important role in bone metabolism and regulates fracture healing in a presently unknown process. In the fracture callus IL-6 expression is biphasic; it peaks during the inflammatory phase and again during intramembranous and endochondral ossification (Ai-Aql et al. 2008). Few studies using IL-6 knockout mice indicate that IL-6 might be crucial for bone healing (Yang et al. 2007). However, a generalized IL-6 knockout induces multi...

ba0005p55 | Bone development/growth and fracture repair | ECTS2016

Calcium and vitamin-D supplementation post-trauma improves bone healing and decreases posttraumatic bone resorption in an osteoporotic mouse model

Heidler Verena , Haffner-Luntzer Melanie , Prystaz Katja , Kroner Jochen , Schinke Thorsten , Amling Michael , Ignatius Anita

Chronic calcium- and vitamin-D-deficiencies are crucial risk factors for osteoporosis. However, their significance for fracture healing is still poorly investigated, despite the clinical evidence that osteoporotic bone healing is disturbed. This study addressed the important question, whether chronic deprivation of calcium and vitamin D compromises bone repair and if this could be rescued by a supplementation post-trauma. Because clinical hints suggest that a fracture induces ...

ba0005p56 | Bone development/growth and fracture repair | ECTS2016

Morphological and densitometric properties of tarsometatarsus in adult male and female emu (Dromaius Novaehollandiae)

Tatara Marcin , Krupski Witold , Charuta Anna , Luszczewska-Sierakowska Iwona , Szczerbinska Danuta , Majewska Danuta , Krzyzanowski Konrad , Brodzki Adam

Considering limited information on skeletal system properties in emu (Dromaius novaehollandiae), the aim of this study was to determine morphological and densitometric parameters of tarsometatarsus obtained from adult males (N=6) and females (N=7). The study was performed on birds reared at the Department of Poultry and Ornamental Bird Breeding of Western Pomeranian University of Technology in Szczecin, Poland. Male and female emu were slaughtered to obtain l...

ba0005p57 | Bone development/growth and fracture repair | ECTS2016

Interrelationships between morphometric, densitometric and mechanical properties of femur in ostriches (Struthio Camelus)

Krupski Witold , Tatara Marcin , Charuta Anna , Brodzki Adam , Luszczewska-Sierakowska Iwona , Polawska Ewa , Jozwik Artur , Strzalkowska Nina , Horbanczuk Jaroslaw

The aim of the study was to evaluate interrelationships between morphometric, densitometric and mechanical properties of femur in ostriches (Struthio camelus). 14-month-old male and female ostriches (N=46) were slaughtered to obtain left femur for analyses. Using quantitative computed tomography (QCT) technique, volumetric bone mineral density (vBMD) of the trabecular (Td) and cortical bone (Cd) were measured. Bone mineral density (BMD) and bone mineral content (BMC) ...

ba0005p58 | Bone development/growth and fracture repair | ECTS2016

Loss of androgen receptor suppresses chondrogenic proliferation during endochondral ossification in mice

Kang Hong-Yo , Chen Yun-Ju , Huang Ko-En , Chang Chawnshang

There have been considerable advances in our knowledge of how loss of androgens signalling leads to bone loss in aging male with hypogonadism. However, the roles of androgen actions on the skeletal growth remain limited. Recently, new insights into the function of and androgen receptor (AR), learned from human genetic mutations and gene targeting mouse models have contributed to emerged understanding of the androgenic effects on bone and cartilage in health and diseases. Here,...

ba0005p59 | Bone development/growth and fracture repair | ECTS2016

Midkine is involved in the pathogenesis of delayed osteoporotic fracture healing after ovariectomy in mice

Haffner-Luntzer Melanie , Kemmler Julia , Heidler Verena , Prystaz Katja , Schinke Thorsten , Amling Michael , Ignatius Anita , Liedert Astrid

Previous studies demonstrated a negative influence of the growth- and differentiation factor midkine (MDK) on bone formation during bone remodeling and fracture healing. Additionally, MDK-deficiency protected mice from a loss of trabecular bone mass after ovariectomy (OVX). Therefore, we hypothesized that MDK may also be involved in the pathogenesis of delayed, osteoporotic fracture healing after OVX in mice. Thus, we analyzed the expression of MDK during bone regeneration and...

ba0005p60 | Bone development/growth and fracture repair | ECTS2016

Role of PHOSPHO1 in chondrocyte matrix vesicle mineralization: an AFM study

Bottini Massimo , Yadav Manisha , Bhattacharya Kunal , Magrini Andrea , Rosato Nicola , Fadeel Bengt , Farquharson Colin , Luis Millan Jose

We used atomic force microscopy (AFM) to study the morphology and development of mineralization-competent matrix vesicles (MVs) secreted by chondrocytes isolated from WT and Phospho1−/− mice in order to validate the role of PHOSPHO1 in MV mineralization. All MVs appeared as flattened globular features either individually dispersed or connected to a mat-like structure. The mat-like structure very closely resembled type-X collagen that has been de...

ba0005p61 | Bone development/growth and fracture repair | ECTS2016

Advanced phase gestational jet lag reduces bone mass of adult offspring

van der Eerden Bram , Chaves Ines , Azimzadeh Pedram , Schreuders-Koedam Marijke , Reiss Irwin , van Leeuwen Johannes , van der Horst Gijsbertus

The mammalian circadian clock is tightly controlled by clock genes, which have been shown to regulate up to 20% of the transcriptome in any given tissue. Evidence is accumulating that light-modulation perpetually affects circadian clock performance. In accordance, shift work or chronic jet lag is associated with higher risk of disease later in life, including osteoporosis. In this study, we assessed whether gestational jet lag in mice reduces bone mass postnatally.<p class...

ba0005p62 | Bone development/growth and fracture repair | ECTS2016

Bcl-2-associated athanogene-1 (BAG-1) regulates chondrocyte and osteoblast development

Greenhough Joanna , Papadakis Emmanouil , Cutress Ramsey , Townsend Paul , Oreffo Richard , Tare Rahul

The co-chaperone, Bcl-2-associated athanogene-1 (BAG-1), is expressed by chondrocytes and osteogenic cells, and interacts via heat shock chaperones (HSC70/HSP70) with diverse proteins such as nuclear hormone receptors to regulate cell proliferation, differentiation and apoptosis. Early embryonic lethality in Bag-1 null mice has limited the investigation of the function of BAG-1 in skeletal development. The present study aimed to elucidate the role of BAG-1 in skeletal...

ba0005p63 | Bone development/growth and fracture repair | ECTS2016

Comparison of bone stiffness during fracture healing in the human distal radius assessed with HR-pQCT using μFEA and FEA based on downscaled images

Voermans Anne , de Jong Joost , Willems Paul , Geusens Piet , van den Bergh Joop , van Rietbergen Bert

High resolution peripheral quantitative computed tomography (HR-pQCT) in combination with micro finite element analysis (μFEA) is a promising tool to assess longitudinal changes in bone mechanical properties during the fracture healing process in the distal radius. In the present study we investigate if these changes can be detected as well when using images with lower resolutions, comparable to clinical QCT images.Postmenopausal women with a stable...

ba0005p64 | Bone development/growth and fracture repair | ECTS2016

A novel model for examining chondral bone regenerative potential in adult zebrafish

Govezensky Yael , David Dalia , Ben-Yosef Dafna , Shochat Chen , Karasik David

Purpose: The aim of this research was to develop an in vivo platform for conveniently examining the potential of various factors to augment chondral bone regeneration. For this purpose, we have established a de novo partial tail amputation model in adult zebrafish, which includes the resection of cartilage-template based bones from the endoskeletal caudal complex. Endoskeletal amputations were rarely studied, as opposed to those of the distal caudal fin rays, a well-e...

ba0005p65 | Bone development/growth and fracture repair | ECTS2016

Effect of postponing puberty and cross-sex hormone therapy on bone turnover markers and BMAD in transgender adolescents

Vlot Mariska , Klink Daniel , den Heijer Martin , Blankenstein Marinus , Rotteveel Joost , Heijboer Annemieke

Background: Puberty is highly important for the accumulation of bone mass. Bone turnover and bone mineral density can be affected in transgender adolescents when puberty is postponed by gonadotropin-releasing hormone analogues (GnRHa), followed by treatment with cross-sex hormone therapy (CSHT).Objective: To investigate the effect of GnRHa and CSHT on bone turnover markers (BTMs) and bone mineral apparent density (BMAD) in transgender adolescents.<p ...

ba0005p66 | Bone development/growth and fracture repair | ECTS2016

OSTEOGROW: BMP6 device for enhance bone healing

Grgurevic Lovorka , Oppermann Hermann , Jankolija Morana , Popek Irena , Erjavec Igor , Dumic-Cule Ivo , Peric Mihaela , Vukicevic Slobodan

High doses of BMPs are needed to achieve clinical success in bone fracture repair with currently available devices. BMP6 has a higher potency in stimulation of bone formation then its paralogue BMP7 due to less susceptibility to Noggin. The BMP6 carrier is a whole blood derived coagulum (WBCD) from the peripheral blood (OSTEOGROW). This WBCD, as an endogenous biocompatible material, significantly reduces the inflammatory response associated with current BMP-based treatments. M...

ba0005p67 | Bone development/growth and fracture repair | ECTS2016

Comparison of osteoconductivity and absorbability of beta-tricalcium phosphate and hydroxyapatite in open wedge high tibial osteotomy

Oh Kwang Jun , Koh Young Bong

The purposes of this present study were to compare the osteoconductivity and absorbability in patients of open wedge high tibial osteotomy with medially gaps filled with hydroxyapatite (HA) or beta-tricalcium phosphate (β-TCP).Total 41 knees of 40 patients in whose follow up period more than 1 year were enrolled. The patients were divides into two groups, group I (22 knees, 21 patients) used HA and group II (19knees, 19 patients) used β-TCP...

ba0005p68 | Bone development/growth and fracture repair | ECTS2016

Adiponectin prevents orthodontic tooth movement in rats

Haugen Sigrid , Matre Aasarod Kristin , Kamilla Stunes Astrid , Peder Mosti Mats , Franzen Tanya , Vandevska-Radunovich Vaska , Syversen Unni , Reseland Janne

Adiponectin may play a role in both bone and periodontal remodeling. In this study, the effect of repetitive local administration of human recombinant adiponectin on experimental tooth movement was examined.The first molar of 24 male Wistar rats was moved mesially by a closed coil spring ligated to the molar and anchored to the incisors in front for 14 days, with adiponectin injections every 3rd day. Amount of tooth movement was examined by feeler gauge ...

ba0005p69 | Bone development/growth and fracture repair | ECTS2016

The effects of bone wound healing by implanting novel type I collagen scaffolds

Kunii Saori , Yamamoto Ei , Akahoshi Yasumitsu , Morimoto Koichi

Background: Three-dimensional scaffolds for the tissue regeneration are also crucial materials for bone formation. In particular, the scaffolds must provide the space for the cell migration and new bone formation. Collagen is one of the useful scaffolds to reach these performance, however, it is not always a desired biodegradability. Recently, we succeeded in developing low adhesive scaffold type I collagen (LASCol) (patent pending) which has the ability to form fibrils. In th...

ba0005p70 | Bone development/growth and fracture repair | ECTS2016

Is heparin effective for the controlled delivery of high dose bone morphogeneic protein-2?

Kim In Sook , Kim Ri Youn , Hwang Soon Jung

Sustained release of bone morphogeneic protein (BMP)-2 by heparin-contained biomaterials is advantageous for bone tissue regeneration using low dose BMP-2. However, its effect in high dose BMP-2 is still unclear and should be clarified considering the clinical use of high dose of BMP-2 in spine and oral surgery. This study aimed to evaluate the efficacy of heparin-conjugated collagen sponge (HCS) in high dose BMP-2 delivery by investigating in vivo initial osteogenic regulatio...

ba0005p71 | Bone development/growth and fracture repair | ECTS2016

Transcriptional analysis of bone healing in mouse: an insight into biological and chronological overlapping genes

Malhan Deeksha , Schmidt-Bleek Katharina , Duda Georg , Heiss Christian , El Khassawna Thaqif

The healing of skeletal fractures involves a cascade of overlapping cellular events. This study aims to deepen the understanding of molecular networks orchestrating these events.Standard closed fracture in the left femur of male 8–10 weeks old C57BL/6N mice were analyzed at (day=D) D3, D7, D10, D14, D21 & D28 post fracture (N=5/time point). Total RNA was prepared for whole genome expression profiling using Illumina μ-array kit. Dat...

ba0005p72 | Bone development/growth and fracture repair | ECTS2016

Sulfated hyaluronan improves bone defect healing in type 2 diabetic rats by increasing osteoblast function

Picke Ann-Kristin , Salbach-Hirsch Juliane , Hintze Vera , Rauner Martina , Moller Stephanie , Kascholke Christian , Hacker Michael C , Schulz-Siegmund Michaela , Schnabelrauch Matthias , Scharnweber Dieter , Hofbauer Lorenz C , Hofbauer Christine

Bone fractures of patients suffering from type 2 diabetes mellitus (T2DM) represent an emerging socioeconomic problem. Underling mechanisms are poorly understood and therapies are limited. Our previous studies have shown that sulfated hyaluronan (sHA3) suppresses osteoclast activity while supporting osteoblast function in vitro. Hence, we now investigated if sHA3 can improve the delayed fracture healing in rats with T2DM.Porous, cross-linked lactide-base...

ba0005p73 | Bone development/growth and fracture repair | ECTS2016

Combination of novel two-photon photopolymerised scaffolds and bioactive elastin-like-recombinamers induce bone regeneration

Kampleitner Carina , Hildebrand Gerhard , Liefeith Klaus , Gonzalez Constancio , Rodriguez-Cabello Jose Carlos , Markus Andrea , Dullin Christian , Alves Frauke , Hoffmann Oskar

Non-healing fractures caused by trauma, disease or tumour resection demand the use of bone grafts to support and stimulate the healing process. We sought to develop and evaluate the effect of a novel bioactive biodegradable biomaterial designed to fill large bone lesions and to improve bone healing. We used two-photon polymerised synthetic polymer scaffolds composed of lactide (LA), caprolactone (CL), and methacrylate (MA) with varying LA: CL ratio and percentage of methacryla...

ba0005p74 | Bone development/growth and fracture repair | ECTS2016

In vitro investigation of the effect of Magnetic Resonance guided Focused Ultrasound Surgery on osteosarcoma cell lines

Agnese Valentina , Costa Viviana , Carina Valeria , Bellavia Daniele , Raimondi Lavinia , De Luca Angela , Scoarughi Gianluca , Midiri Massimo , Stassi Giorgio , Giavaresi Gianluca

Aim: Magnetic Resonance guided Focused Ultrasound Surgery (MRgFUS) is an invasive treatment able to control local disease and pain of bone tumors. Unfortunately, there is not any scientific evidence of the biological effect of MRgFUS treatment on tumor cells, especially in lower dose region, where tissues are only warmed to sub-lethal temperatures. Here we investigate the effect of in vitro MRgFUS treatment, at different levels of acoustic energy (200–630 J), on ...

ba0005p75 | Bone development/growth and fracture repair | ECTS2016

Increased periosteal expansion, Osterix expression and osteogenic potential upon bone injury during perturbed PI3K signaling

Walia Bhavita , Scanlon Vanessa , Yu Jungeun , Maye Peter , Drissi Hicham , Sanjay Archana

Periosteum contains mesenchymal progenitors and is essential for fracture healing. Signaling mechanisms governing periosteal reaction to injury remain largely unidentified. We previously investigated how PI3 kinase signaling affects the skeletal system using CblYF/YF knock-in (YF) mice wherein PI3K signaling is perturbed by abolition of interaction between Cbl, an E3 ubiquitin-ligase/adaptor protein, and p85 subunit of PI3K. YF mice displayed increased bone volume u...

ba0005p76 | Bone development/growth and fracture repair | ECTS2016

Multicentre study reveals poor correlation between in vitro and in vivo assessments of biomaterials for bone-regeneration

Billstrom Gry Hulsart , Dawson Jon , Hofmann Sandra , Muller Ralph , Stoddart Martin , Alini Mauro , Redl Heinz , Haj Alicia El , Brown Robert , Salih Vehid , Hilborn Jons , Larsson Sune , Oreffo Richard

Introduction: Research on biomaterials for bone regeneration generates a plethora of new biomaterials requiring evaluation with reliable and comparable methods of biocompatibility and functionality for clinical translation. To reduce the burden of in vivo assessment, there is a need for refined in vitro assays that are predictive of in vivo outcomes. This retrospective study correlated in vitro results with in vivo outcomes observed...

ba0005p77 | Bone development/growth and fracture repair | ECTS2016

The high bone volume phenotype of female nNOS KO mice is not maintained with ageing

van't Hof Rob , Rose Lorraine , Charlesworth Gemma , Prior Amanda , Daroszewska Anna

We have previously shown that female neuronal nitric oxide synthase knockout (nNOS KO) mice have increased trabecular bone volume. However, this study was performed in mice at 10 weeks of age only. To investigate whether the high bone volume is maintained during ageing, we compared 3-month- and 12-month-old wild type (WT) and nNOS KO mice using μCT. The tibias from 8 WT and 8 nNOS KO mice at each age were dissected, fixed for 24 h in buffered formalin, stored in 70% ethan...