Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp70 | Bone development/growth and fracture repair | ECTS2013

A casein-based diet leads to a better bone status than a soy protein-based diet during moderate protein restriction in growing mice

Rouy Emilien , Laroche Norbert , Blachier Francois , Tome Daniel , Vico Laurence , Blais Anne

This study aims at determining if casein would lead to a better bone status than soy in the context of a moderate protein restriction (6% of total energy intake) in growing mice.Ten-week-old female Balb/C mice were divided in four groups of 15 animals. Two groups received 6% of their energy intake as protein, one as casein and the other as soy protein. The third group was a normal-protein control receiving 20% soy protein. The last group (positive contro...

ba0003pp127 | Cell biology: osteoblasts and bone formation | ECTS2014

Regulation of adipo- and osteo-genesis of multipotent cells by strontium through stimulation of small Rho GTPases: A 3D bioreactor study

Louis Fiona , Linossier Marie-Therese , Peyroche Sylvie , Vico Laurence , Guignandon Alain

Small GTPases of the Rho family (RhoA and Rac-1) are responsible for cytoskeleton dynamics (particularly actin polymerisation) and control cellular tension. For these reasons, they are implicated in the commitment of multipotent cells (MCs). In one hand, increased tension (important RhoA activity) is commonly associated with osteogenesis (OS), in the other hand, a reduced one (low RhoA activity) is associated with adipogenesis (AD). Nevertheless, precise RhoGTPases regulations...

ba0001pp181 | Cell biology: osteoblasts and bone formation | ECTS2013

The impairement of bone formation and mineralization in BSP−/− mouse calvaria cell cultures is partly rescued by increasing cell density

Bouet Guenaelle , Bouleftour Wafa , David Marchat , Marie-Therese Linossier , Mireille Thomas , Jane Aubin E , Laurence Vico , Luc Malaval

Bone sialoprotein regulates osteoblast activity and bone formation. In knockout (BSP−/−) mouse bone marrow (BM) stromal cell cultures, the pool of osteoprogenitor (OP) cells (CFU-F number) is not different from wild-type (+/+), nor is their early differentiation (same numbers of alkaline phosphatase positive colonies=CFU-ALP, although these are smaller), while the number of osteoblast, mineralized colonies (CFU-OB) is dramatically reduced. Because ossifi...

ba0003pp39 | Bone development/growth and fracture repair | ECTS2014

Bone shaft revascularization after marrow ablation is dramatically accelerated in BSP−/− mice, along with faster haematopoietic recolonization

Bouleftour Wafa , Granito Renata Neves , Bossche Arnaud Vanden , Sabido Odile , Roche Bernard , Thomas Mireille , Linossier Marie-Therese , Aubin Jane , Lafage-Proust Marie-Helene , Vico Laurence , Malaval Luc

The bone organ integrates the activity of bone tissue, bone marrow, and blood vessels and the factors ensuring this coordination remain ill defined. Bone sialoprotein (BSP) is with osteopontin (OPN) a member of the small integrin binding ligand N-linked glycoprotein (SIBLING) family, involved in bone formation, hematopoiesis, and angiogenesis. In rodents, bone marrow ablation induces a rapid formation of medullary bone which peaks by ~8 days (d8) and is blunted in BSP...

ba0005p31 | Bone biomechanics and quality | ECTS2016

Site specific bone loss induced by spaceflight in mice: a multiscale evaluation of trabecular and cortical structure and quality

Gerbaix Maude , Gnyubkin Vasily , Delphine Farlay , Helene Follet , Patrick Ammann , Cecile Olivier , Francoise Peyrin , Norbert Laroche , Boris Shenkman , Guillemette Gauquelin-Koch , Laurence Vico

Intrinsic quality, micro and nano structure of bone after 1 month of spaceflight and during 1 week of recovery had never been investigated. The aim was to explore these properties in appendicular and axial skeleton of mature mice.Ten C57/Bl6 male mice flew on the 30-day space Russian BION-M1 high-orbit satellite mission (Biomedical Ethics Commission of IBMP, n°319). Five were euthanized 12 h after landing, the others 8 days later. A ground control g...