Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp63 | Bone development/growth and fracture repair | ECTS2013

Microtomography analysis of bone formation in calvarial defects filled with gelatin sponge or fibrin glue

de Santana Santos Thiago , Lopes Helena Bacha , Almeida Adriana Luiza , Beloti Marcio Mateus , Rosa Adalberto Luiz

Bone tissue engineering relies on the combination of scaffolds and cells aiming to heal bone defects. Among the many candidates for scaffolds, gelatin sponge (Gelfoam®) and fibrin glue (Tissucol®) are of interest due to the well-established biocompatibility and the ability to be loaded with cells. Here, we evaluated their potential to stimulate bone formation by microtomography analysis. For this, 5-mm unilateral calvarial defect was created in rats deeply anesthetiz...

ba0005p152 | Cell biology: osteoblasts and bone formation | ECTS2016

Participation of microRNA-34a/RANKL in the osteogenic potential of the Poly(vinylidene-trifluorethylene)/barium titanate membrane

Lopes Helena Bacha , Ferraz Emanuela Prado , de Almeida Adriana Luisa Goncalves , Abuna Rodrigo Paolo Flores , Hassan Mohammad Quamurul , Rosa Adalberto Luiz , Beloti Marcio Mateus

Barrier membranes have been extensively used in dentistry to prevent soft tissue down-growth into the bone defects and to promote alveolar ridge augmentation. Previous studies of our group showed that the Poly(vinylidene-trifluoroethylene)/barium titanate composite (PVDF) enhances both the in vitro osteoblastic differentiation and the in vivo bone repair compared with a commercially available polytetrafluoroethylene (PTFE) membrane. As bone formation may be regulated ...

ba0003pp119 | Cell biology: osteoblasts and bone formation | ECTS2014

Effect of osteoporosis in the transcriptional profile of osteoblastic cells from bone marrow and calvaria of ovariectomized rats

Semeghini Mayara Sgarbi , de Azevedo Fernanda Grilo , de Assis Amanda Freire , Siessere Selma , Beloti Marcio Mateus , Rosa Adalberto Luiz , Fernandes Roger Rodrigo , Passos Geraldo Aleixo , Prado Karina Fittipaldi Bombonato

Changes in the ability of self-renewal and differentiation of mesenchymal stem cells may be affected by osteoporosis. The aim of this investigation was to compare the transcriptional profile of mRNAs and miRNAs of osteoblastic cells from calvaria and bone marrow of female rats after ovariectomy. Following the approval by ethics committee, 18 wistar rats were divided into control (sham) and ovariectomized groups. After 150 days, both groups were sacrificed to collect the femurs...