Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp19 | Arthritis and other joint diseases: translational and clinical | ECTS2013

Vitamin K2 administration is associated with decreased disease activity in patients with rheumatoid arthritis

Ebina Kosuke , Morimoto Tokimitsu , Shi Kenrin , Kaneshiro Shoichi , Koizumi Kota , Hirao Makoto , Hashimoto Jun , Yoshikawa Hideki

Objectives: Recent studies have demonstrated that vitamin K2 (VitK2) induces apoptosis of not only osteoclasts but also rheumatoid arthritis (RA) synovial cells in vitro, while its clinical effect on disease activity of RA remains unknown.Methods: 158 female RA patients (age 62.5 years, duration of disease 14.9 years) who had not been treated with warfarin, biologics, or teriparatide were enrolled in this study. VitK2 (45 mg/day) was administered orally ...

ba0001pp148 | Cancer and bone: basic, translational and clinical | ECTS2013

Direct administration of zoledronate acid improves bone structure in local osteoporotic lesion of ovariectomized rats

Matsuo Yohei , Kashii Masafumi , Sugiura Tsuyoshi , Morimoto Tokimitsu , Honda Hirotsugu , Kaito Takashi , Iwasaki Motoki , Yoshikawa Hideki

Objective: To examine the efficacy and safety of direct administration of zoledronate acid (ZOL) on local osteoporotic lesion of ovariectomized rats.Methods: Six weeks later after ovariectomy, 16 6-month-old female S.D. rats were divided into the two groups with no differences of body weight and BMD of the proximal tibia. In the group L, 50 μl ZOL at a dose of 67 μg/kg were locally injected into the bone marrow between the two dr...

ba0001pp258 | Chondrocytes and cartilage | ECTS2013

Transcription factor Nkx3.2 plays crucial role in primary chondrogenesis by up-regulating type II collagen a1 transcription activity

Ebina Kosuke , Kawato Yoshitaka , Hirao Makoto , Honjo Yui , Morimoto Tokimitsu , Hashimoto Jun , Shi Kenrin , Yoshikawa Hideki

Objectives: Sox9 is a dominant but insufficient transcription factor to induce thorough primary chondrogenesis, so other factors which may induce primary chondrogenesis besides Sox9 have been assumed. The previously reported function of transcription factor Nkx3.2 is to maintain chondrogenic phenotype by suppressing Runx2, while recent studies demonstrated that mouse Nkx3.2 null mice shows severe metaphyseal dysplasia which is similar to that seen in type II collagen a1 (Col2a...

ba0001pp412 | Osteoporosis: treatment | ECTS2013

Preoperative bisphosphonate treatment in patients with neuromuscular scoliosis improves bone strength of vertebral body

Kashii Masafumi , Nagamoto Yukitaka , Fujimori Takahito , Honda Hirotsugu , Kaito Takashi , Yoshikawa Hideki , Iwasaki Motoki

Background: Boys with muscular dystrophy as presented by Duchenne muscular dystrophy (DMD) lose muscle strength and are usually confined to a wheelchair until 13 years. Furthermore they often have development of myogenic scoliosis, and scoliosis surgery is necessary to acquire sitting balance. Osteoporosis is one of the major concerns to perform surgical treatment. Patients with DMD or congenital muscular dystrophy (CMD) have fragile bones due to loss of ambulation, glucocorti...

ba0005p50 | Bone development/growth and fracture repair | ECTS2016

Prostaglandin IP agonist promotes osteoblastic differentiation and BMP induced bone formation

Kanayama Sadaaki , Kaito Takashi , Kashii Masafumi , Makino Takahiro , Morimoto Tokimitsu , Kitaguchi Kazuma , Ishiguro Hiroyuki , Yoshikawa Hideki

Introduction: A synthetic prostacyclin IP receptor agonist (ONO-1301) has been reported to induce the production of endogenous HGF and VEGF in fibroblasts by stimulating cAMP production.Materials and methods: In vitro; murine primary osteoblasts and cell line (ST2, MC3T3-E1, C2C12) were treated with BMP-2 (0–100 ng/ml) and ONO-1301 (0–10−6 M). ALP assay and cell proliferation assay were performed. In vivo analysis, Col...

ba0005p52 | Bone development/growth and fracture repair | ECTS2016

Effect of intermittent administration of teriparatide (PTH 1-34) on BMP induced bone regeneration in a rat critical-sized femoral defect model

Kanayama Sadaaki , Kaito Takashi , Kashii Masafumi , Makino Takahiro , Morimoto Tokimitsu , Kitaguchi Kazuma , Ishiguro Hiroyuki , Yoshikawa Hideki

Introduction: Indirect inhibition of the extracellular BMP antagonist network and the activation of Wnt signaling by PTH1-34 suggest the possibility of synergistic effect on bone regeneration by the co-administration of BMP and PTH1-34.Materials and methods: In a rat critical-sized femoral defect (7 mm) model, SD rats (n=32) were operated by absorbable collagen sponge containing two different BMP-2 dosage treatments; 1) 2 μg (low dose) and ...

ba0001pp478 | Other diseases of bone and mineral metabolism | ECTS2013

Circulating sclerostin level in patients with ossification of the posterior longitudinal ligament of the spine

Kashii Masafumi , Matsuo Yohei , Sugiura Tsuyoshi , Fujimori Takahito , Nagamoto Yukitaka , Honda Hirotsugu , Kaito Takashi , Iwasaki Motoki , Yoshikawa Hideki

Backgrounds: Ossification of the posterior longitudinal ligament (OPLL) is characterized by pathological ectopic ossification of the posterior longitudinal ligament. Development of OPLL induces compression myelopathy or radiculopathy by spinal stenosis and the loss of spinal flexibility by ankylosing spinal hyperosteosis (ASH). Although the etiology of OPLL has not been fully elucidated, systemic and local bone formation factors may play a role in the pathogenesis of OPLL. The...

ba0004p11 | (1) | ICCBH2015

Effects of bisphosphonate for the development of scoliosis in children with osteogenesis imperfecta

Kashii Masafumi , Kanayama Sadaaki , Kitaoka Taichi , Makino Takahiro , Kaito Takashi , Kubota Takuo , Namba Noriyuki , Yamamoto Takehisa , Ozono Keiichi , Yoshikawa Hideki

Backgrounds: Osteogenesis imperfecta (OI) is an inherited bone disease caused by qualitative or quantitative defects in type I collagen, and is characterized by bone fragility and ligamentous laxity. Spine disorder is an important symptom in children with OI, and respiratory difficulties secondary to spinal disorder were identified as a main cause of death in these patients. Reduced fracture rates and prevention of long-bone deformities have been reported in children with OI w...

ba0005p49 | Bone development/growth and fracture repair | ECTS2016

The effects of combined teriparatide and denosumab on bone regeneration

Kitaguchi Kazuma , Kashii Masafumi , Sugiura Tsuyoshi , Tokimitsu Morimoto , Noguchi Takaaki , Makino Takahiro , Ebina Kosuke , Kaito Takashi , Yoshikawa Hideki

Objective: The purpose of this study is to investigate the effects of the combined teriparatide (TPTD) and denosumab (DMAB) on both cortical and cancellous bone regeneration.Materials and methods: Bone defects were created in the diaphysis of the right femur and in the epimetaphysis of the left femur in 8-week old female C57/BL6N mice. After making bone defects, Mice were given either saline or 40 μg/kg TPTD for 20 days (5× per week) and were s...