Searchable abstracts of presentations at key conferences on calcified tissues

ba0004is24 | (1) (1) | ICCBH2015

The new histology

Misof Barbara M

Additionally to the standard histomorphometric and pathohistologic evaluation, the transiliac bone biopsy sample from a patient can be further analyzed for additional information on structural and material characteristics. Under the term ‘new histology’, several non-destructive techniques and combinations thereof providing high spatial resolution have been introduced. In contrast to conventional histology, these techniques allow the characterization of structural, co...

ba0004is24biog | (1) (1) | ICCBH2015

The new histology

Misof Barbara M

Biographical DetailsBarbara M Misof is Staff Scientist at the Ludwig Boltzmann Institute of Osteology, Vienna, Austria. She completed her PhD in Physics and Postgraduate Education in Medical Physics at the University of Vienna in 2000. In 2002, she received the Herbert-Czitober-Research Award of the Austrian Society of Bone and Mineral Research. Her areas of interest are bone material pro...

ba0003pp22 | Bone biomechanics and quality | ECTS2014

Bone matrix mineralization is preserved during perimenopausal stage in healthy women

Misof Barbara , Roschger Paul , Recker Robert , Klaushofer Klaus

Menopause is accompanied by a decrease in bone mineral density which can be caused by a reduction in bone volume and/or degree of bone matrix mineralization. Both of them are suggested to contribute to the increased fracture risk in postmenopausal individuals. In the present work, we aimed for information whether a drop in bone matrix mineralization is occurring in the perimenopausal stage of women. For this purpose, we measured the bone mineralization density distribution (BM...

ba0004oc4 | (1) | ICCBH2015

Increased bone matrix mineralization in treatment-naive children with Crohn's disease

Roschger Paul , Misof Barbara , Klaushofer Klaus , Rauch Frank , Ward Leanne

Background: Crohn’s disease (CD) is an inflammatory bowel disease which affects many organ systems including the skeleton. In children with CD, bone mineral density is frequently low. Bone metabolic abnormalities, including lower biochemical measures of bone turnover (NTX) as well as decreased bone formation indices at the tissue level have been reported. The aim of our study was to gain information on the bone matrix mineralization in CD.Methods: W...

ba0003pp25 | Bone biomechanics and quality | ECTS2014

Normal bone matrix mineralization in patients with chronic obstructive pulmonary disease

Misof Barbara , Roschger Paul , Jorgetti Vanda , Klaushofer Klaus , Dempster David , Kulak Carolina

Chronic obstructive pulmonary disease (COPD) has been found associated with low areal bone mineral density and an increase in fracture rate. Previous histomorphometric findings revealed abnormally low cancellous bone volume and thin cortices. In the present work, we studied the same transiliac bone biopsy samples from n=19 COPD patients for cancellous (Cn.) and cortical (Ct.) bone mineralization density distribution (BMDD) based on quantitative backscatter electron im...

ba0003pp359 | Osteoporosis: treatment | ECTS2014

Ten year alendronate use does not adversely affect bone quality compared to 5 years use: a human iliac crest biopsy study

Hassler Norbert , Gamsjaeger Sonja , Hofstetter Birgit , Brozek Wolfgang , Misof Barbara , Roschger Paul , Klaushofer Klaus , Paschalis Eleftherios

Bisphosphonates (BPs) including alendronate (ALN) are the most widely prescribed therapy for post-menopausal osteoporosis. Despite their overall excellent safety record and efficacy in reducing fractures, concerns have been expressed lately regarding potential detrimental effects due to prolonged bone turnover reduction, although no definite cause–effect relationship has been established to date. The purpose of the present study was to determine bone material quality by R...

ba0003pp407 | Paediatric bone disease | ECTS2014

Glucocorticoid-treated boys with Duchenne muscular dystrophy DMD and osteoporosis have higher bone matrix mineralization before and after i.v. bisphosphonate therapy

Roschger Paul , Misof Barbara M. , Klaushofer Klaus , Rauch Frank , Ward Leanne M

Duchenne muscular dystrophy (DMD) causes progressive muscle weakness and loss of ambulation. While glucocorticoid (GC) therapy improves motor function, many boys sustain fractures due to osteoporosis. Recently, i.v. bisphosphonate (i.v.BP) therapy has shown promise in the treatment of DMD-related osteoporosis. At the same time, bone histomorphometry revealed lowered bone volume and significant reductions in bone formation pre-i.v.BP treatment, and a further drop after 2 years&...

ba0007p169 | (1) | ICCBH2019

Rescue diet restores bone matrix mineralization in mice with a non-functioning vitamin D receptor

Misof Barbara , Blouin Stephane , Hartmann Markus , Hofstaetter Jochen , Klaushofer Klaus , Zwerina Jochen , Roschger Paul , Erben Reinhold

Objectives: Mice with a non-functioning vitamin D receptor (VDR mutants) develop severe secondary hyperparathyroidism, which can be rescued by a diet enriched with calcium, phosphate and lactose. In this work, we studied the effects of a low calcium challenge (CD), normal calcium (ND) and a calcium enriched rescue diet (RD) on the bone mineralization density distribution (BMDD) and osteocyte lacunae sections (OLS) in these mice.Methods: BMDD and OLS were...

ba0002op7 | (1) | ICCBH2013

Bone mineralization density distribution, bone formation rate and bone turnover markers in a cohort of children with CKD before and after GH treatment

Fratzl-Zelman Nadja , Nawrot-Wawrzyniak Kamilla , Misof Barbara , Panczyk-Tomaszewska Malgorzata , Ziolkowska Helena , Roschger Paul , Klaushofer Klaus

Background and methods: A specific skeletal complication in children with CKD is growth impairment. The effects of recombinant human growth hormone (rhGH) therapy on bone turnover and bone material quality are not well understood. Bone matrix mineralization density distribution (BMDD) as assessed by quantitative backscattered electron microscopy (qBEI) is an important determinant of bone material quality and reflects bone turnover (average tissue age) and mineralization kineti...