Searchable abstracts of presentations at key conferences on calcified tissues

ba0003pp171 | Cell biology: osteoclasts and bone resorption | ECTS2014

The purinergenic receptor P2Y14 is essential for RANKL-induced osteoclastogenesis

Park Jin Hee , Jung Eutteum , Lee Soo Young

The P2Y14 (purinergic receptor P2Y, G protein coupled, 14) receptor for UDP-glucose and other UDP-sugars has been implicated in the regulation of the stem cell compartment as well as neuroimmune function. However, the role of P2Y14 in osteoclast formation is completely unknown. We found that RANKL selectively induced P2Y14 among seven mammalian P2Y receptors when analysed at both the mRNA and protein level, but inhibitors of the MAP pathway suppressed induction of P2Y14 protei...

ba0007p24 | (1) | ICCBH2019

The role of the RACK1-c-Src axis in regulation of osteoclast function

Park Jin Hee , Jeong Eutteum , Lee Soo Young

Activation of p38 MAPK by RANKL is regulated by the scaffolding protein receptor for activated C-kinase 1 (RACK1) in osteoclast precursors, whereas it is unclear whether RACK1 can also affect in mature osteoclasts. In this study, to identify that the interaction of RACK1 with c-Src is essential for osteoclast function, we generated several mutants affecting the RACK1-c-Src association. A RACK1 mutant protein (mutations of tyrosine 228 and 246 residues to phenylalanine) disrupt...

ba0003pp160 | Cell biology: osteoclasts and bone resorption | ECTS2014

EEIG1 interacts with RANK receptor and positively regulates osteoclastogenesis

Jung Eutteum , Park Jin Hee , Choi Han Kyung , Lee Soo Young

The receptor activator of NF-kB (RANK) and ITAM-containing adaptors have been identified as essential factors involved in osteoclast formation and bone remodeling, but their mechanisms and interacting factors have not yet been fully identified. Here we report that early estrogen-induced gene 1 (EEIG1), a novel RANK ligand (RANKL)-inducible protein, physically interacts with RANK and further associates with Gab2, PLCg2, and Tec/Btk kinases by RANKL stimulation. Overexpression o...