Searchable abstracts of presentations at key conferences on calcified tissues

ba0005p71 | Bone development/growth and fracture repair | ECTS2016

Transcriptional analysis of bone healing in mouse: an insight into biological and chronological overlapping genes

Malhan Deeksha , Schmidt-Bleek Katharina , Duda Georg , Heiss Christian , El Khassawna Thaqif

The healing of skeletal fractures involves a cascade of overlapping cellular events. This study aims to deepen the understanding of molecular networks orchestrating these events.Standard closed fracture in the left femur of male 8–10 weeks old C57BL/6N mice were analyzed at (day=D) D3, D7, D10, D14, D21 & D28 post fracture (N=5/time point). Total RNA was prepared for whole genome expression profiling using Illumina μ-array kit. Dat...

ba0005p314 | Osteoporosis: evaluation and imaging | ECTS2016

Inducing osteoporosis in a large animal model: influence of ovariectomy, diet and corticoid therapy

Sender Jonas , El Khassawna Thaqif , Erdmann Georg , Bocker Wolfgang , Krombach Gabriele A. , Langheinrich Alexander C. , Kampschulte Marian

Purpose: The aim of our study is a micro-CT-based, longitudinal characterisation of ovariectomy, multideficient diet and corticoid therapy impact on the ossal microarchitecture.Materials and methods: Four randomized groups were formed out of 32 adult female merino land sheep:1. control2. ovariectomized (OVX)3. OVX+multideficient diet (OVX-D)4. OVX-D+320 mg methylp...

ba0005p270 | Muscle, physical activity and bone | ECTS2016

A new tapping screw design for anterior cruciate ligament reconstruction

El Khassawna Thaqif , Engelhardt Markus , Eldin Daghma Diaa , Malhan Deeksha , Kern Stefanie , Tushtev Kamen , Rezwan Kurosch , Hein Sebastian , Haack Janne , Morino Sergio , Wagner Ulrich , Heiss Christian

Interference fit fixation of soft-tissue grafts is in clinical focus to allow anatomic graft fixation which increases stability and graft isometry. Although clinical data show promising results with different screw materials, the issue of biodegradability, handling and stability in the first weeks remain under demand. The purpose of the present study was to investigate anterior cruciate ligament (ACL) reconstruction radiologically, biomechanically and histologically using dire...

ba0001pp454 | Other diseases of bone and mineral metabolism | ECTS2013

Long bone fragility in NF1 is due to deficiency of architecture, micro-structure and matrix mineralization

Kuhnisch Jirko , Seto Jong , Lange Claudia , Schrof Susanne , Stumpp Sabine , Kobus Karolina , Grohmann Julia , Kossler Nadine , Varga Peter , Osswald Monika , Tinschert Sigrid , Seifert Wenke , el Khassawna Thaqif , Stevenson David , Elefteriou Florent , Kornak Uwe , Raum Kay , Fratzl Peter , Kolanczyk Mateusz , Mundlos Stefan

Neurofibromatosis type I (NF1) is a monogenetic disorder caused by mutations in the NF1 gene encoding for the Ras-GAP protein neurofibromin. Apart from benign tumour development NF1 is frequently associated with skeletal manifestations such as osteopenia or debilitating focal skeletal dysplasia. To assess a function of Nf1 in osteocytes we here apply a combinatorial approach of biophysical, histological and molecular techniques allowing differential analysis ...