Searchable abstracts of presentations at key conferences on calcified tissues

ba0001pp63 | Bone development/growth and fracture repair | ECTS2013

Microtomography analysis of bone formation in calvarial defects filled with gelatin sponge or fibrin glue

de Santana Santos Thiago , Lopes Helena Bacha , Almeida Adriana Luiza , Beloti Marcio Mateus , Rosa Adalberto Luiz

Bone tissue engineering relies on the combination of scaffolds and cells aiming to heal bone defects. Among the many candidates for scaffolds, gelatin sponge (Gelfoam®) and fibrin glue (Tissucol®) are of interest due to the well-established biocompatibility and the ability to be loaded with cells. Here, we evaluated their potential to stimulate bone formation by microtomography analysis. For this, 5-mm unilateral calvarial defect was created in rats deeply anesthetiz...

ba0005p142 | Cell biology: osteoblasts and bone formation | ECTS2016

Regulation of genes involved in the integrin signalling pathway induced by titanium with nanotopography

Lopes Helena , Freitas Gileade , Florio Pedro , de Oliveira Paulo , Rosa Adalberto , Beloti Marcio

Titanium (Ti) surface with nanotopography induces osteoblast differentiation, at least in part, by upregulating the expression of α1/β1 integrins. Thus, we hypothesized that other potential candidates from the integrin signalling pathway may be involved in the osteogenic potential of nanotopography. In this context, the aim of this study was to compare the expression of integrin family members of cells grown on nanotopography with machined surface. Machined Ti discs ...

ba0005p53 | Bone development/growth and fracture repair | ECTS2016

Effect of bioactive glass-ceramic scaffold associated with bone marrow - or adipose-derived mesenchymal stem cells on bone formation under osteoporotic conditions

Freitas Gileade , Lopes Helena , Almeida Adriana , de Souza Luiz , Siessere Selma , Regalo Simone , Beloti Marcio , Rosa Adalberto

In this study, we evaluated the effect of the association of a bioactive glass-ceramic scaffold (Biosca) with mesenchymal stem cells derived from either bone marrow (BM-MSCs) or adipose tissue (AT-MSCs) on bone formation in calvarial defects of osteoporotic rats. Wistar rats were submitted to bilateral ovariectomy (OVX) or only to the surgical stress (Sham), under approval of the Committee of Ethics in Animal Research. After 5 months, 5-mm unilateral calvarial defect was creat...

ba0005p152 | Cell biology: osteoblasts and bone formation | ECTS2016

Participation of microRNA-34a/RANKL in the osteogenic potential of the Poly(vinylidene-trifluorethylene)/barium titanate membrane

Lopes Helena Bacha , Ferraz Emanuela Prado , de Almeida Adriana Luisa Goncalves , Abuna Rodrigo Paolo Flores , Hassan Mohammad Quamurul , Rosa Adalberto Luiz , Beloti Marcio Mateus

Barrier membranes have been extensively used in dentistry to prevent soft tissue down-growth into the bone defects and to promote alveolar ridge augmentation. Previous studies of our group showed that the Poly(vinylidene-trifluoroethylene)/barium titanate composite (PVDF) enhances both the in vitro osteoblastic differentiation and the in vivo bone repair compared with a commercially available polytetrafluoroethylene (PTFE) membrane. As bone formation may be regulated ...