Searchable abstracts of presentations at key conferences on calcified tissues

ba0001oc2.3 | Bone quality and fracture repair - animal models | ECTS2013

Low-magnitude high-frequency vibration improves fracture healing in aged, ovariectomized mice

Wehrle Esther , Bindl Ronny , Wehner Tim , Heilmann Aline , Fischer Lena , Noland Jarrod , Amling Michael , Ignatius Anita

Introduction: Fracture healing is impaired in aged and osteoporotic patients. Because bone formation is tightly regulated by the mechanical conditions in the fracture gap and because suitable mechanical stimuli improve fracture healing (Claes et al. 1998), we investigated whether low-magnitude high-frequency vibration (LMHFV; Rubin et al. 2004) is able to improve delayed fracture healing induced by age and ovariectomy in mice.Sudy desig...

ba0001pp83 | Bone development/growth and fracture repair | ECTS2013

Trabecular micro-architecture of the proximal femur during post-natal growth

Djuric Marija , Milovanovic Petar , Djonic Danijela , Hahn Michael , Busse Bjoern , Amling Michael

In sharp contrast to the great interest in morphology of the proximal femur in aged individuals, there is a paucity of studies in children. To date, a comprehensive quantitative analysis of trabecular micro-architecture in various biomechanically relevant subregions of the immature proximal femur has been lacking. The aim of this study was to characterize postnatal development of trabecular bone in various regions of the human proximal femur. The study sample was comprised of ...

ba0001pp302 | Muscle, physical activity and bone | ECTS2013

Morphology of muscle attachment sites and microarhitecture of underlying bone as the markers of physical activities of past populations

Djukic Ksenija , Milovanovic Petar , Hahn Michael , Busse Bjoern , Amling Michael , Djuric Marija

Habitual physical activities of past populations are frequently reconstructed based on musculoskeletal markers (MSM) in human skeletal remains, i.e. particular morphological features of muscles attachment sites. However, the relationship between muscular activity and bone microstructure at the site of muscle attachments is unexplored. Therefore, this study aimed at analyzing bone microstructural characteristics of the muscles attachments sites and correlating these features wi...

ba0001pp317 | Osteoporosis: evaluation and imaging | ECTS2013

A 3D QCT technique of the thoracic and lumbar spine: integral volume and intervertebral disc space increase and bmd decreases from T6 to L4

Museyko Oleg , Heinemann Axel , Krause Mattias , Barkmann Reinhard , Amling Michael , Gluer Claus , Puschel Klaus , Engelke Klaus

Introduction: QCT of the spine is typically restricted to the BMD analysis of the lumbar vertebrae. However, fractures frequently occur in the thoracolumbar region. Also the load distribution in the spine may depend on the intervertebral disc space (IDS), a good approximation of the intervertebral disc, which itself cannot be reliably assessed by X-ray based methods.Materials and methods: A QCT 3D acquisition and automated analysis technique (with option...

ba0003pp46 | Bone development/growth and fracture repair | ECTS2014

Midkine deficiency significantly delayed chondrogenesis during fracture healing in mice

Haffner-Luntzer Melanie , Heilmann Aline , Rapp Anna , Schinke Thorsten , Amling Michael , Ignatius Anita , Liedert Astrid

One growth factor that potentially plays a role in fracture healing is midkine (Mdk). Mdk is expressed in chondrocytes during bone repair (Ohta et al. 1999) and has been shown to influence bone mass and mechanotransduction (Neunaber et al. 2010, Liedert et al. 2011). The aim of the present study was to evaluate the effects of Mdk-deficiency on bone repair in a standardized mouse femur osteotomy model.Mdk-deficient and wildtype ...

ba0005p28 | Bone biomechanics and quality | ECTS2016

Alendronate therapy improves anterior vertebral microstructure in osteoporotic bone facilitating fracture risk reduction

vom Scheidt Annika , Krause Matthias , Puschel Klaus , Amling Michael , Busse Bjorn

Although the fracture risk reduction under bisphosphonate treatment in osteoporosis is clinically well established, it remains understudied why the efficacy of bisphosphonate therapy is higher for the prevention of vertebral fractures compared to other fractures.Our aim was to investigate whether anti-resorptive therapy with alendronate would result in different regional variations of structural indices in the vertebral body. We investigated the microstr...

ba0005p33 | Bone biomechanics and quality | ECTS2016

Assessment of changes in collagen associated with advanced glycation end-products in human bone using vibrational spectroscopy

Schmidt Felix Nikolai , Zimmermann Elizabeth Ann , Puschel Klaus , Amling Michael , Vashishth Deepka , Tang Simon , Busse Bjorn

Introduction: Aging, diabetes and other disease conditions are associated with the accumulation of non-enzymatic collagen cross-links (NE-XL) in human tissues. Non-enzymatic cross-links (i.e. advanced glycation end products (AGEs)), occur at the bone collagen level, where they are associated with individuals increased fracture risk caused by bone’s reduced plasticity.Methods: Here, non-destructive Fourier-transform infrared (FTIR) spectroscopy was u...

ba0005p55 | Bone development/growth and fracture repair | ECTS2016

Calcium and vitamin-D supplementation post-trauma improves bone healing and decreases posttraumatic bone resorption in an osteoporotic mouse model

Heidler Verena , Haffner-Luntzer Melanie , Prystaz Katja , Kroner Jochen , Schinke Thorsten , Amling Michael , Ignatius Anita

Chronic calcium- and vitamin-D-deficiencies are crucial risk factors for osteoporosis. However, their significance for fracture healing is still poorly investigated, despite the clinical evidence that osteoporotic bone healing is disturbed. This study addressed the important question, whether chronic deprivation of calcium and vitamin D compromises bone repair and if this could be rescued by a supplementation post-trauma. Because clinical hints suggest that a fracture induces ...

ba0001pp51 | Bone biomechanics and quality | ECTS2013

Microstructural adaptation of bone tissue of the facial skeleton to the distribution of occlusal load under physiological conditions

Janovic Aleksa , Milovanovic Petar , Saveljic Igor , Nikolic Dalibor , Hahn Michael , Busse Bjoern , Rakocevic Zoran , Filipovic Nenad , Amling Michael , Djuric Marija

Despite widely accepted classical mechanical theory of buttresses as a load bearing areas in the midfacial skeleton, the data related to its microstructural adaptation on functional demands are scarce. In this study we investigated microstructural features of the different regions in the facial bones in relation to the occlusal load dissipation using a combination of finite element analysis (FEA) and micro-CT analysis (μCT). The FEA was performed on the model of the dry h...